ArticleFASEB journal : official publication of the Federation of American Societies for Experimental Biology2011
Determining the fate of seeded cells in venous tissue-engineered vascular grafts using serial MRI.
Article in FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers, 1 of them a synthesis that pooled it.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
30 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Pooled it
- Functional integration of an autologous engineered esophagus in a large-animal model.Nature biotechnology · 2026Article
- In-Situ Vascular Regeneration by Host Cells of Acellular Human Saphenous Vein Implanted in Porcine Carotid Artery.International journal of molecular sciences · 2025Article
- Recent advances in artificial intelligent strategies for tissue engineering and regenerative medicine.Skin research and technology : official journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI) · 2024Review
- Hemodynamics and Wall Mechanics of Vascular Graft Failure.Arteriosclerosis, thrombosis, and vascular biology · 2024Review
- Tissue engineering: Relevance to neonatal congenital heart disease.Seminars in fetal & neonatal medicine · 2022Review
- Tissue engineered vascular grafts transform into autologous neovessels capable of native function and growth.Communications medicine · 2022Article
- Application of medical imaging methods and artificial intelligence in tissue engineering and organ-on-a-chip.Frontiers in bioengineering and biotechnology · 2022Review
- Surface modification of decellularized bovine carotid arteries with human vascular cells significantly reduces their thrombogenicity.Journal of biological engineering · 2021Article
- Iron Oxide Nanoparticles in Regenerative Medicine and Tissue Engineering.Nanomaterials (Basel, Switzerland) · 2021Review
- Systematic Review of Tissue-Engineered Vascular Grafts.Frontiers in bioengineering and biotechnology · 2021Review
- Tissue Engineered Vascular Graft Recipient Interleukin 10 Status Is Critical for Preventing Thrombosis.Advanced healthcare materials · 2020Article
- Spontaneous reversal of stenosis in tissue-engineered vascular grafts.Science translational medicine · 2020Article
- The Evolution of Tissue Engineered Vascular Graft Technologies: From Preclinical Trials to Advancing Patient Care.Applied sciences (Basel, Switzerland) · 2019Article
- Implantation of VEGF-functionalized cell-free vascular grafts: regenerative and immunological response.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Magnetic Resonance Imaging of Shear Stress and Wall Thickness in Tissue-Engineered Vascular Grafts.Tissue engineering. Part C, Methods · 2018Article
- The Heart and Great Vessels.Cold Spring Harbor perspectives in medicine · 2018Review
- The role of myeloid cell-derived PDGF-B in neotissue formation in a tissue-engineered vascular graft.Regenerative medicine · 2017Article
- Biomaterial-driven in situ cardiovascular tissue engineering-a multi-disciplinary perspective.NPJ Regenerative medicine · 2017Review
- Deconstructing the Tissue Engineered Vascular Graft: Evaluating Scaffold Pre-Wetting, Conditioned Media Incubation, and Determining the Optimal Mononuclear Cell Source.ACS biomaterials science & engineering · 2017Article
Corrections and comments
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Authors and funding
15 authors.
Funding
Abstract
A major limitation of tissue engineering research is the lack of noninvasive monitoring techniques for observations of dynamic changes in single tissue-engineered constructs. We use cellular magnetic resonance imaging (MRI) to track the fate of cells seeded onto functional tissue-engineered vascular grafts (TEVGs) through serial imaging. After in vitro optimization, murine macrophages were labeled with ultrasmall superparamagnetic iron oxide (USPIO) nanoparticles and seeded onto scaffolds that were surgically implanted as inferior vena cava interposition grafts in SCID/bg mice. Serial MRI showed the transverse relaxation times (T(2)) were significantly lower immediately following implantation of USPIO-labeled scaffolds (T(2) = 44 ± 6.8 vs. 71 ± 10.2 ms) but increased rapidly at 2 h to values identical to control implants seeded with unlabeled macrophages (T(2) = 63 ± 12 vs. 63 ± 14 ms). This strongly indicates the rapid loss of seeded cells from the scaffolds, a finding verified using Prussian blue staining for iron containing macrophages on explanted TEVGs. Our results support a novel paradigm where seeded cells are rapidly lost from implanted scaffolds instead of developing into cells of the neovessel, as traditionally thought. Our findings confirm and validate this paradigm shift while demonstrating the first successful application of noninvasive MRI for serial study of cellular-level processes in tissue engineering.
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Registered trials
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