ArticleThe Journal of biological chemistry2011
Association of ActA to peptidoglycan revealed by cell wall proteomics of intracellular Listeria monocytogenes.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 49 citations in OpenAlex.
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- Assessment of Listeria monocytogenes Surface Proteins Identified from Proteomics Analysis for Use as Diagnostic Biomarkers.Applied and environmental microbiology · 2022Article
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- Switching Lifestyles Is anFrontiers in cellular and infection microbiology · 2019Review
- Intracellular/surface moonlighting proteins that aid in the attachment of gut microbiota to the host.AIMS microbiology · 2019Review
- Chitinase Expression in Listeria monocytogenes Is Influenced byApplied and environmental microbiology · 2017Article
- Proteogenomics in Aid of Host-Pathogen Interaction Studies: A Bacterial Perspective.Proteomes · 2017Review
- Listeria monocytogenes encodes a functional ESX-1 secretion system whose expression is detrimental to in vivo infection.Virulence · 2017Article
- Comparative genomics of human and non-human Listeria monocytogenes sequence type 121 strains.PloS one · 2017Article
- The Listeria Small RNA Rli27 Regulates a Cell Wall Protein inside Eukaryotic Cells by Targeting a Long 5'-UTR Variant.PLoS genetics · 2014Article
- How Listeria monocytogenes organizes its surface for virulence.Frontiers in cellular and infection microbiology · 2014Review
- Non-coding RNA regulation in pathogenic bacteria located inside eukaryotic cells.Frontiers in cellular and infection microbiology · 2014Review
- Ultra deep sequencing of Listeria monocytogenes sRNA transcriptome revealed new antisense RNAs.PloS one · 2014Article
- How do bacteria localize proteins to the cell pole?Journal of cell science · 2014Review
- An RpoS-dependent sRNA regulates the expression of a chaperone involved in protein folding.RNA (New York, N.Y.) · 2013Article
- Remodeling of the Listeria monocytogenes cell wall inside eukaryotic cells.Communicative & integrative biology · 2012Article
- Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Listeria monocytogenes is a Gram-positive intracellular bacterial pathogen that colonizes the cytosol of eukaryotic cells. Recent transcriptomic studies have revealed that intracellular L. monocytogenes alter expression of genes encoding envelope components. However, no comparative global analysis of this cell wall remodeling process is yet known at the protein level. Here, we used high resolution mass spectrometry to define the cell wall proteome of L. monocytogenes growing inside epithelial cells. When compared with extracellular bacteria growing in a nutrient-rich medium, a major difference found in the proteome was the presence of the actin assembly-inducing protein ActA in peptidoglycan purified from intracellular bacteria. ActA was also identified in the peptidoglycan of extracellular bacteria growing in a chemically defined minimal medium. In this condition, ActA maintains its membrane anchoring domain and promotes efficient bacterial entry into nonphagocytic host cells. Unexpectedly, Internalin-A, which mediates entry of extracellular L. monocytogenes into eukaryotic cells, was identified at late infection times (6 h) as an abundant protein in the cell wall of intracellular bacteria. Other surface proteins covalently bound to the peptidoglycan, as Lmo0514 and Lmo2085, were detected exclusively in intracellular and extracellular bacteria, respectively. Altogether, these data provide the first insights into the changes occurring at the protein level in the L. monocytogenes cell wall as the pathogen transits from the extracellular environment to an intracytosolic lifestyle inside eukaryotic cells. Some of these changes include alterations in the relative amount and the mode of association of certain surface proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.