ArticleInternational journal of legal medicine2012
Validation of reference genes for estimating wound age in contused rat skeletal muscle by quantitative real-time PCR.
Article in International journal of legal medicine, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 44 citations in OpenAlex.
- State-of-the-Art on Wound Vitality Evaluation: A Systematic Review.International journal of molecular sciences · 2022Pooled it
- Novel ratio-expressions of genes enables estimation of wound age in contused skeletal muscle.International journal of legal medicine · 2024Article
- Novel Prediction Method Applied to Wound Age Estimation: Developing a Stacking Ensemble Model to Improve Predictive Performance Based on Multi-mRNA.Diagnostics (Basel, Switzerland) · 2023Article
- Screening criteria of mRNA indicators for wound age estimation.Forensic sciences research · 2022Article
- Novel insights into wound age estimation: combined with "up, no change, or down" system and cosine similarity in python environment.International journal of legal medicine · 2020Article
- Temporal expression of wound healing-related genes inform wound age estimation in rats after a skeletal muscle contusion: a multivariate statistical model analysis.International journal of legal medicine · 2020Article
- Article
- Vitality and wound-age estimation in forensic pathology: review and future prospects.Forensic sciences research · 2020Review
- Article
- Selection of suitable endogenous reference genes for qPCR in kidney and hypothalamus of rats under testosterone influence.PloS one · 2017Article
- Daily rhythmicity of clock gene transcript levels in fast and slow muscle fibers from Chinese perch (Siniperca chuatsi).BMC genomics · 2016Article
- Immunohistochemical detection of intrathrombotic IL-6 and its application to thrombus age estimation.International journal of legal medicine · 2015Article
- Selection of reference genes for expression analyses in liver of rats with impaired glucose metabolism.International journal of clinical and experimental pathology · 2015Article
- Reference gene selection for real-time RT-PCR normalization in rice field eel (Monopterus albus) during gonad development.Fish physiology and biochemistry · 2014Article
- Time-dependent expression of SNAT2 mRNA in the contused skeletal muscle of rats: a possible marker for wound age estimation.Forensic science, medicine, and pathology · 2013Article
- Immunohistochemical detection of intrathrombotic macrophage-derived cytokines and its application to thrombus age estimation in murine deep vein thrombosis model.International journal of legal medicine · 2013Article
- Molecular pathology of brain edema after severe burns in forensic autopsy cases with special regard to the importance of reference gene selection.International journal of legal medicine · 2013Article
- Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gene expression profiling by quantitative real-time PCR (RT-qPCR) is a valuable tool in forensic science for estimating the age of a wound. To accurately assess gene expression levels over time in injured tissue, the genes used as internal reference standards must be carefully validated for transcriptional stability. This study examined the transcriptional stability of nine potential reference genes (β-actin, GAPDH, RPL32, PGK1, SDHA, RPL13, HPRT, Tbp, and Ywhaz) in contused rat skeletal muscle by RT-qPCR. The raw Ct values were determined for each candidate gene at different time points following contusion, and the data were analyzed by the NormFinder, geNorm, and BestKeeper validation programs. The reference genes RPL13 and RPL32 were the most stably expressed genes in contused skeletal muscle, whereas PGK1 was the least stable. The commonly used reference genes β-actin and GAPDH appeared to be too unstable for normalization of RT-qPCR expression profiling in contused muscle. The reference genes RPL13 and RPL32 were also the best combination for multianalysis. The use of RPL13 and RPL32 as internal standards may improve the accuracy of gene expression studies aimed at determining the age of early wounds in forensic investigations.
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