ArticleInfection and immunity2011
The novel leptospiral surface adhesin Lsa20 binds laminin and human plasminogen and is probably expressed during infection.
Article in Infection and immunity, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 67 citations in OpenAlex.
- DMEM and EMEM as alternate growth media for pathogenic Leptospira.PLoS neglected tropical diseases · 2026Article
- In vitro and in vivo endothelial interactions of Leptospira species are markers of virulence.PLoS neglected tropical diseases · 2026Article
- Evaluation of binding activities of a putative lipoprotein LIC_13355 of Leptospira spp.FEBS open bio · 2025Article
- DMEM and EMEM are suitable surrogate media to mimic host environment and expand leptospiral pathogenesis studies usingbioRxiv : the preprint server for biology · 2025Article
- Leptospiral adhesins: from identification to future perspectives.Frontiers in microbiology · 2024Review
- A Review on Host-Frontiers in cellular and infection microbiology · 2021Review
- The interaction of two novel putative proteins ofVirulence · 2019Article
- What Makes a Bacterial Species Pathogenic?:Comparative Genomic Analysis of the Genus Leptospira.PLoS neglected tropical diseases · 2016Article
- Complement Evasion by PathogenicFrontiers in immunology · 2016Review
- Protective Immunity and Reduced Renal Colonization Induced by Vaccines Containing Recombinant Leptospira interrogans Outer Membrane Proteins and Flagellin Adjuvant.Clinical and vaccine immunology : CVI · 2015Article
- Evaluation of cell binding activities of Leptospira ECM adhesins.PLoS neglected tropical diseases · 2015Article
- Features of two new proteins with OmpA-like domains identified in the genome sequences of Leptospira interrogans.PloS one · 2015Article
- Description of a novel adhesin of Mycobacterium avium subsp. paratuberculosis.BioMed research international · 2014Article
- Leptospira interrogans binds to cadherins.PLoS neglected tropical diseases · 2014Article
- Characterization of three novel adhesins of Leptospira interrogans.The American journal of tropical medicine and hygiene · 2013Article
- BBA70 of Borrelia burgdorferi is a novel plasminogen-binding protein.The Journal of biological chemistry · 2013Article
- Interaction of Leptospira interrogans with human proteolytic systems enhances dissemination through endothelial cells and protease levels.Infection and immunity · 2013Article
- Adhesins of Leptospira interrogans mediate the interaction to fibrinogen and inhibit fibrin clot formation in vitro.PLoS neglected tropical diseases · 2013Article
- LipL32 Is a Subsurface Lipoprotein of Leptospira interrogans: presentation of new data and reevaluation of previous studies.PloS one · 2013Article
- Leptospiral outer membrane protein microarray, a novel approach to identification of host ligand-binding proteins.Journal of bacteriology · 2012Article
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Leptospirosis is an emerging infectious disease caused by pathogenic species of Leptospira. In this work, we report the cloning, expression, purification, and characterization of two predicted leptospiral outer membrane proteins, LIC11469 and LIC11030. The LIC11469 protein is well conserved among leptospiral strains, while LIC11030 was identified only in Leptospira interrogans. We confirmed by surface proteolysis of intact leptospires with proteinase K that these proteins are most likely new surface leptospiral proteins. The recombinant proteins were evaluated for their capacity to attach to extracellular matrix (ECM) components and to plasminogen. The leptospiral protein encoded by LIC11469, named Lsa20 (leptospiral surface adhesin of 20 kDa), binds to laminin and to plasminogen. The binding with both components was not detected when Lsa20 was previously denatured or blocked with anti-Lsa20 antibodies. Moreover, Lsa20 binding to laminin was also confirmed by surface plasmon resonance (SPR). Laminin competes with plasminogen for binding to Lsa20, suggesting the same ligand-binding site. Lsa20-bound plasminogen could be converted to enzymatically active plasmin, capable of cleaving plasmin substrate d-valyl-leucyl-lysine-p-nitroanilide dihydrochloride. Lsa20 was recognized by antibodies in confirmed-leptospirosis serum samples, suggesting that this protein is expressed during infection. Taken together, our results indicate that Lsa20 is a novel leptospiral adhesin that in concert with the host-derived plasmin may help the bacteria to adhere and to spread through the hosts.
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