ArticleThe Journal of cell biology2011
Actomyosin II contractility expels von Willebrand factor from Weibel-Palade bodies during exocytosis.
Article in The Journal of cell biology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 78 papers.
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Who cites it
78 citing papers in PubMed.
- Endothelial β-PIX (ARHGEF7) supports actomyosin mediated expulsion of VWF through dynamic reorganization of the cytoskeleton.Molecular biology of the cell · 2026Article
- RAB5C Increases Endothelial Release of VWF by Regulating Vesicle Trafficking.Arteriosclerosis, thrombosis, and vascular biology · 2026Article
- PI(4,5)PBiochimica et biophysica acta. Molecular and cell biology of lipids · 2025Review
- Weibel-Palade bodies - secretory organelles at the interface of inflammation and hemostasis.Frontiers in cell and developmental biology · 2025Review
- Coordination of force-generating actin-based modules stabilizes and remodels membranes in vivo.The Journal of cell biology · 2024Article
- Myosin-1C augments endothelial secretion of von Willebrand factor by linking contractile actomyosin machinery to the plasma membrane.Blood advances · 2024Article
- Myo1c drives actin-dependent VWF expulsion from EC WPBs.Blood advances · 2024Article
- Patterning of the cell cortex by Rho GTPases.Nature reviews. Molecular cell biology · 2024Review
- Thrombomodulin Regulates PTEN/AKT Signaling Axis in Endothelial Cells.Arteriosclerosis, thrombosis, and vascular biology · 2024Article
- Membrane transformations of fusion and budding.Nature communications · 2024Review
- Fusion pore dynamics of large secretory vesicles define a distinct mechanism of exocytosis.The Journal of cell biology · 2023Article
- Cooperative cell-cell actin network remodeling to perform Gap junction endocytosis.Basic and clinical andrology · 2023Article
- Swip-1 promotes exocytosis of glue granules in the exocrine Drosophila salivary gland.Journal of cell science · 2023Article
- Article
- Fibroblasts secrete fibronectin under lamellipodia in a microtubule- and myosin II-dependent fashion.The Journal of cell biology · 2023Article
- Dispatch and delivery at the ER-Golgi interface: how endothelial cells tune their hemostatic response.The FEBS journal · 2022Article
- Tip-end fusion of a rod-shaped secretory organelle.Cellular and molecular life sciences : CMLS · 2022Article
- Spire1 and Myosin Vc promote CaCellular and molecular life sciences : CMLS · 2022Article
- Acidification of endothelial Weibel-Palade bodies is mediated by the vacuolar-type H+-ATPase.PloS one · 2022Article
- A novel function for Rab1 and Rab11 during secretory granule maturation.Journal of cell science · 2021Article
18 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
The study of actin in regulated exocytosis has a long history with many different results in numerous systems. A major limitation on identifying precise mechanisms has been the paucity of experimental systems in which actin function has been directly assessed alongside granule content release at distinct steps of exocytosis of a single secretory organelle with sufficient spatiotemporal resolution. Using dual-color confocal microscopy and correlative electron microscopy in human endothelial cells, we visually distinguished two sequential steps of secretagogue-stimulated exocytosis: fusion of individual secretory granules (Weibel-Palade bodies [WPBs]) and subsequent expulsion of von Willebrand factor (VWF) content. Based on our observations, we conclude that for fusion, WPBs are released from cellular sites of actin anchorage. However, once fused, a dynamic ring of actin filaments and myosin II forms around the granule, and actomyosin II contractility squeezes VWF content out into the extracellular environment. This study therefore demonstrates how discrete actin cytoskeleton functions within a single cellular system explain actin filament-based prevention and promotion of specific exocytic steps during regulated secretion.
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