ArticleBiophysical journal2011
The growth of sickle hemoglobin polymers.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Voxelotor does not inhibit sickle hemoglobin fiber formation upon complete deoxygenation.Biophysical journal · 2023Article
- Fluorescence Lifetime Measurement of Prefibrillar Sickle Hemoglobin Oligomers as a Platform for Drug Discovery in Sickle Cell Disease.Biomacromolecules · 2022Article
- An Experimental-Computational Approach to Quantify Blood Rheology in Sickle Cell Disease.Biophysical journal · 2020Article
- Quantitative prediction of erythrocyte sickling for the development of advanced sickle cell therapies.Science advances · 2019Article
- Molecular insights into the irreversible mechanical behavior of sickle hemoglobin.Journal of biomolecular structure & dynamics · 2019Article
- Rapid and inefficient kinetics of sickle hemoglobin fiber growth.Science advances · 2019Article
- Cooperative Assembly of Hsp70 Subdomain Clusters.Biochemistry · 2018Article
- Mesoscopic Adaptive Resolution Scheme toward Understanding of Interactions between Sickle Cell Fibers.Biophysical journal · 2017Article
- Probing the Twisted Structure of Sickle Hemoglobin Fibers via Particle Simulations.Biophysical journal · 2016Article
- Ratchets, red cells, and metastability.Biophysical reviews · 2013Review
- Multifunctional magnetic rotator for micro and nanorheological studies.The Review of scientific instruments · 2012Article
- Predicting the morphology of sickle red blood cells using coarse-grained models of intracellular aligned hemoglobin polymers.Soft matter · 2012Article
- Nucleation of sickle hemoglobin mixed with hemoglobin A: experimental and theoretical studies of hybrid-forming mixtures.Biophysical journal · 2011Article
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Authors and funding
3 authors.
Funding
Abstract
The measurement of polymer growth is an essential element in characterization of assembly. We have developed a precise method of measuring the growth of sickle hemoglobin polymers by observing the time required for polymers to traverse a photolytically produced channel between a region in which polymers are created and a detection region. The presence of the polymer is functionally detected by observing its ability to create new polymers through the well-established process of heterogeneous nucleation. Using this method, we have determined the rate constants for monomer addition to and release from polymer ends, as well as their temperature dependences. At 25°C we find k(+) = 84 ± 2 mM⁻¹ s⁻¹ and k(-) = 790 ± 80 molecules/s from each end. These numbers are in accord with differential interference contrast measurements, and their ratio gives a solubility measured on individual fibers. The single-fiber solubility agrees with that measured in sedimentation experiments. The concentration dependence of the monomer addition rate is consistent with monomer addition, but not oligomer addition, to growing polymers. The concentration dependence suggests the presence of an activation enthalpy barrier, and the rate of monomer addition is not diffusion-limited. Analysis of the temperature dependence of the monomer addition rate reveals an apparent activation energy of 9.1 ± 0.6 kcal/mol.
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