ArticleBiophysical journal2011
PicoNewton-millisecond force steps reveal the transition kinetics and mechanism of the double-stranded DNA elongation.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 31 citations in OpenAlex.
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- Matching Mechanics and Energetics of Muscle Contraction Suggests Unconventional Chemomechanical Coupling during the Actin-Myosin Interaction.International journal of molecular sciences · 2023Article
- Optical manipulation: advances for biophotonics in the 21st century.Journal of biomedical optics · 2021Review
- A Myosin II-Based Nanomachine Devised for the Study of CaInternational journal of molecular sciences · 2020Article
- Single-molecule studies of high-mobility group B architectural DNA bending proteins.Biophysical reviews · 2017Review
- An AT-barrier mechanically controls DNA reannealing under tension.Nucleic acids research · 2016Article
- A HaloTag Anchored Ruler for Week-Long Studies of Protein Dynamics.Journal of the American Chemical Society · 2016Article
- Equilibrium and kinetics of DNA overstretching modeled with a quartic energy landscape.Biophysical journal · 2014Article
- The transition mechanism of DNA overstretching: a microscopic view using molecular dynamics.Journal of the Royal Society, Interface · 2014Article
- Force-induced melting of DNA--evidence for peeling and internal melting from force spectra on short synthetic duplex sequences.Nucleic acids research · 2014Article
- Transient kinetics measured with force steps discriminate between double-stranded DNA elongation and melting and define the reaction energetics.Nucleic acids research · 2014Article
- Interrogating biology with force: single molecule high-resolution measurements with optical tweezers.Biophysical journal · 2013Review
- Single-molecule kinetics reveal microscopic mechanism by which High-Mobility Group B proteins alter DNA flexibility.Nucleic acids research · 2013Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We study the kinetics of the overstretching transition in λ-phage double-stranded (ds) DNA from the basic conformation (B state) to the 1.7-times longer and partially unwound conformation (S state), using the dual-laser optical tweezers under force-clamp conditions at 25°C. The unprecedented resolution of our piezo servo-system, which can impose millisecond force steps of 0.5-2 pN, reveals the exponential character of the elongation kinetics and allows us to test the two-state nature of the B-S transition mechanism. By analyzing the load-dependence of the rate constant of the elongation, we find that the elementary elongation step is 5.85 nm, indicating a cooperativity of ~25 basepairs. This mechanism increases the free energy for the elementary reaction to ~94 k(B)T, accounting for the stability of the basic conformation of DNA, and explains why ds-DNA can remain in equilibrium as it overstretches.
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