ArticleBiophysical journal2011
Phase-separation and domain-formation in cholesterol-sphingomyelin mixture: pulse-EPR oxygen probing.
Article in Biophysical journal, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Preventing Age-Related Nuclear Cataract Development - Is Cholesterol the Key?Investigative ophthalmology & visual science · 2026Review
- Temperature and cholesterol regulate the association of native bovine lens alpha-crystallin with in vitro reconstituted membranes made of synthetically prepared lipids and cholesterol.Experimental eye research · 2026Article
- Association of Alpha-Crystallin with Human Cortical and Nuclear Lens Lipid Membrane Increases with the Grade of Cortical and Nuclear Cataract.International journal of molecular sciences · 2024Article
- Cholesterol Content Regulates the Interaction of αA-, αB-, and α-Crystallin with the Model of Human Lens-Lipid Membranes.International journal of molecular sciences · 2024Article
- Binding of βInternational journal of molecular sciences · 2023Article
- Article
- Binding of Alpha-Crystallin to Cortical and Nuclear Lens Lipid Membranes Derived from a Single Lens.International journal of molecular sciences · 2022Article
- Molecular oxygen as a probe molecule in EPR spin-labeling studies of membrane structure and dynamics.Oxygen (Basel, Switzerland) · 2022Article
- Membrane elasticity modulated by cholesterol in model of porcine eye lens-lipid membrane.Experimental eye research · 2022Article
- Multilamellar Liposomes as a Model for Biological Membranes: Saturation Recovery EPR Spin-Labeling Studies.Membranes · 2022Review
- Precise Detection and Visualization of Nanoscale Temporal Confinement in Single-Molecule Tracking Analysis.Membranes · 2022Article
- Alpha-Crystallin Association with the Model of Human and Animal Eye Lens-Lipid Membranes is Modulated by Surface Hydrophobicity of Membranes.Current eye research · 2022Article
- Alpha-Crystallin-Membrane Association Modulated by Phospholipid Acyl Chain Length and Degree of Unsaturation.Membranes · 2022Article
- Mechanical properties of the high cholesterol-containing membrane: An AFM study.Biochimica et biophysica acta. Biomembranes · 2021Article
- Association of Alpha-Crystallin with Fiber Cell Plasma Membrane of the Eye Lens Accompanied by Light Scattering and Cataract Formation.Membranes · 2021Review
- Cholesterol and cholesterol bilayer domains inhibit binding of alpha-crystallin to the membranes made of the major phospholipids of eye lens fiber cell plasma membranes.Experimental eye research · 2021Article
- Confocal Microscopy Confirmed that in Phosphatidylcholine Giant Unilamellar Vesicles with very High Cholesterol Content Pure Cholesterol Bilayer Domains Form.Cell biochemistry and biophysics · 2019Article
- High Cholesterol/Low Cholesterol: Effects in Biological Membranes: A Review.Cell biochemistry and biophysics · 2017Review
- Selective Membrane Disruption Mechanism of an Antibacterial γ-AApeptide Defined by EPR Spectroscopy.Biophysical journal · 2016Article
- Amounts of phospholipids and cholesterol in lipid domains formed in intact lens membranes: Methodology development and its application to studies of porcine lens membranes.Experimental eye research · 2015Article
Corrections and comments
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Authors and funding
3 authors.
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Abstract
Membranes made of Chol/ESM (cholesterol/egg sphingomyelin) mixtures were investigated using saturation-recovery electron paramagnetic resonance spin-labeling methods, in which bimolecular collisions of relaxation agents (oxygen or nickel ethylenediamine diacetic acid) with spin labels are measured. Liquid-disordered (l(d)) and liquid-ordered (l(o)) phases, and cholesterol bilayer domains (CBDs) were discriminated and characterized by profiles of the oxygen transport parameter (OTP). In the l(d) phase, coexisting with the l(o) phase, the OTP profile is bell-shaped and lies above that in the pure ESM membrane. Changes in the OTP profile across the l(o) phase are complex. When the l(o) phase coexists with the l(d) phase, the OTP profile is similar to that across the pure ESM membrane but with a steeper bell shape. With an increase in cholesterol concentration (up to the cholesterol-solubility threshold), the profile becomes rectangular, with low OTP values from the membrane surface to the depth of C9, and high values in the membrane center. This approximately threefold increase in the OTP occurs at the depth at which the rigid ring structure of cholesterol is immersed. Further addition of cholesterol and the formation of the CBD does not affect the OTP profile across the l(o) phase. OTP values in the CBD are significantly lower than in the l(o) phase.
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