ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2012
Rapid detection of Salmonella sp. by means of a combination of selective enrichment broth and MALDI-TOF MS.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Non-typhoidal salmonella contamination along the pork value chain in a rural East African setting: a cross-sectional study.Transactions of the Royal Society of Tropical Medicine and Hygiene · 2023Article
- Review
- Performance of MALDI-TOF Mass Spectrometry (VITEK MS) in the Identification ofMicroorganisms · 2022Article
- Reducing time in detection ofJournal of food science and technology · 2021Article
- Bacteriophages as Potential Tools for Detection and Control ofMicroorganisms · 2019Review
- A Moldy Application of MALDI: MALDI-ToF Mass Spectrometry for Fungal Identification.Journal of fungi (Basel, Switzerland) · 2019Review
- Detection of Foodborne Pathogens Using Proteomics and Metabolomics-Based Approaches.Frontiers in microbiology · 2018Article
- Recent and emerging innovations in Salmonella detection: a food and environmental perspective.Microbial biotechnology · 2016Review
- Salmonella, Shigella, and yersinia.Clinics in laboratory medicine · 2015Review
- MALDI-TOF mass spectrometry: an emerging technology for microbial identification and diagnosis.Frontiers in microbiology · 2015Review
- Beyond the matrix-assisted laser desorption ionization (MALDI) biotyping workflow: in search of microorganism-specific tryptic peptides enabling discrimination of subspecies.Applied and environmental microbiology · 2014Article
- Subtype determination of Blastocystis isolates by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MALDI-TOF MS).European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2014Article
- The construction and evaluation of reference spectra for the identification of human pathogenic microorganisms by MALDI-TOF MS.PloS one · 2014Article
- A challenge and an opportunity to improve patient management and public health surveillance for food-borne infections through culture-independent diagnostics.Journal of clinical microbiology · 2013Article
- Matrix-assisted laser desorption ionization-time of flight mass spectrometry: a fundamental shift in the routine practice of clinical microbiology.Clinical microbiology reviews · 2013Review
- Application of MALDI-TOF MS for the Identification of Food Borne Bacteria.The open microbiology journal · 2013Article
- Comparison of an in-house method and the commercial Sepsityper™ kit for bacterial identification directly from positive blood culture broths by matrix-assisted laser desorption-ionisation time-of-flight mass spectrometry.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2012Article
- Rapid discrimination of Salmonella enterica serovar Typhi from other serovars by MALDI-TOF mass spectrometry.PloS one · 2012Article
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Authors and funding
4 authors.
Funding
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Abstract
The identification of Salmonella sp. in stool samples usually takes 2 days when employing routine procedures. Fast approaches are necessary in order to shorten the analysis time. The aim of this work was the development of a rapid procedure for the detection of Salmonella sp. from clinical stool samples. Spiked stool samples were cultured in selective selenite enrichment broth. Identifications were directly performed from the liquid broth by the MALDI Biotyper. After the evaluation of this method, the same procedure was applied to clinical samples. Coevally, the samples were streaked on Hektoen agar and single colonies were analyzed by the MALDI Biotyper. For comparison, the liquid broth was plated according to the standard laboratory procedure. A total of 4,847 samples were analyzed for Salmonella sp. In total, 108 Salmonella sp.-positive samples were identified; 66 of these were identified after the streaking of stool samples on Hektoen agar and subsequent MALDI Biotyper analysis of Salmonella sp. suspicious colonies. These and a further 34 samples were detected as Salmonella sp.-positive directly from the selenite enrichment broth on day one. Eight Salmonella sp.-positive samples were not detected before plating of the selenite broth and subsequent MALDI Biotyper analysis on day two. The combination of MALDI Biotyper analysis and selective selenite enrichment broth identification delivers positive results for the majority of the samples already after one day.
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