ArticleThe Journal of biological chemistry2011
The rickettsia surface cell antigen 4 applies mimicry to bind to and activate vinculin.
Article in The Journal of biological chemistry, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed, 69 citations in OpenAlex.
- A conserved interaction between the effector Sca4 and host clathrin suggests additional contributions for Sca4 during rickettsial infection.Infection and immunity · 2024Article
- IpaA reveals distinct modes of vinculin activation duringLife science alliance · 2024Article
- Article
- Signatures in in vitro infection of NSC-34 mouse neurons and their cell nucleus with Rickettsia helvetica.BMC microbiology · 2023Article
- New insights into the impact of microbiome on horizontal and vertical transmission of a tick-borne pathogen.Microbiome · 2023Article
- Metagenome diversity illuminates origins of pathogen effectors.bioRxiv : the preprint server for biology · 2023Article
- Pathogenicity and virulence ofVirulence · 2022Review
- Subversion of host cell signaling: The arsenal of Rickettsial species.Frontiers in cellular and infection microbiology · 2022Review
- Review
- The enigmatic biology of rickettsiae: recent advances, open questions and outlook.Pathogens and disease · 2021Review
- Rickettsia-host interaction: strategies of intracytosolic host colonization.Pathogens and disease · 2021Review
- Manipulation of Focal Adhesion Signaling by Pathogenic Microbes.International journal of molecular sciences · 2021Review
- The Cryogenic Electron Microscopy Structure of the Cell Adhesion Regulator Metavinculin Reveals an Isoform-Specific Kinked Helix in Its Cytoskeleton Binding Domain.International journal of molecular sciences · 2021Article
- Article
- The neglected challenge: Vaccination against rickettsiae.PLoS neglected tropical diseases · 2020Review
- ELM-the eukaryotic linear motif resource in 2020.Nucleic acids research · 2020Article
- Pathogenesis of Rickettsial Diseases: Pathogenic and Immune Mechanisms of an Endotheliotropic Infection.Annual review of pathology · 2019Review
- Chlamydial virulence factor TarP mimics talin to disrupt the talin-vinculin complex.FEBS letters · 2018Article
- In vitro studies of Rickettsia-host cell interactions: Confocal laser scanning microscopy of Rickettsia helvetica-infected eukaryotic cell lines.PLoS neglected tropical diseases · 2018Article
- Engineering of obligate intracellular bacteria: progress, challenges and paradigms.Nature reviews. Microbiology · 2017Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pathogenic Rickettsia species cause high morbidity and mortality, especially R. prowazekii, the causative agent of typhus. Like many intracellular pathogens, Rickettsia exploit the cytoskeleton to enter and spread within the host cell. Here we report that the cell surface antigen sca4 of Rickettsia co-localizes with vinculin in cells at sites of focal adhesions in sca4-transfected cells and that sca4 binds to and activates vinculin through two vinculin binding sites (VBSs) that are conserved across all Rickettsia. Remarkably, this occurs through molecular mimicry of the vinculin-talin interaction that is also seen with the IpaA invasin of the intracellular pathogen Shigella, where binding of these VBSs to the vinculin seven-helix bundle head domain (Vh1) displaces intramolecular interactions with the vinculin tail domain that normally clamp vinculin in an inactive state. Finally, the vinculin·sca4-VBS crystal structures reveal that vinculin adopts a new conformation when bound to the C-terminal VBS of sca4. Collectively, our data define the mechanism by which sca4 activates vinculin and interacts with the actin cytoskeleton, and they suggest important roles for vinculin in Rickettsia pathogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.