ArticleEukaryotic cell2011
Unexpected role for a serine/threonine-rich domain in the Candida albicans Iff protein family.
Article in Eukaryotic cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 44 citations in OpenAlex.
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- Mucosal passive immunization with monoclonal antibodies targeting Candidalysin and Hyr proteins attenuates vaginal candidiasis in mice.Virulence · 2025Article
- Evolutionary accumulation ofEmerging microbes & infections · 2024Article
- To each its own: Mechanisms of cross-talk between GPI biosynthesis and cAMP-PKA signaling in Candida albicans versus Saccharomyces cerevisiae.The Journal of biological chemistry · 2024Review
- Surface adherence and vacuolar internalization of bacterial pathogens to the Candida spp. cells: Mechanism of persistence and propagation.Journal of advanced research · 2023Review
- Ustilago maydis PR-1-like protein has evolved two distinct domains for dual virulence activities.Nature communications · 2023Article
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- Phenotypic and genetic features of a novel clinically isolated rough morphotypeFrontiers in microbiology · 2023Article
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- Functional Divergence in a Multi-gene Family Is a Key Evolutionary Innovation for Anaerobic Growth in Saccharomyces cerevisiae.Molecular biology and evolution · 2022Article
- A novel class of Candida glabrata cell wall proteins with β-helix fold mediates adhesion in clinical isolates.PLoS pathogens · 2021Article
- Using Genomics to Shape the Definition of the Agglutinin-Like Sequence (Frontiers in cellular and infection microbiology · 2021Article
- Moonlighting Proteins at the Candidal Cell Surface.Microorganisms · 2020Review
- CaNRT2.1 Is Required for Nitrate but Not Nitrite Uptake in Chili Pepper PathogenFrontiers in microbiology · 2020Article
- Agglutinin-Like Sequence (Frontiers in microbiology · 2019Article
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- Biological Roles of Protein-Coding Tandem Repeats in the YeastJournal of fungi (Basel, Switzerland) · 2018Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Glycosylphosphatidylinositol (GPI)-anchored proteins are an important class of cell wall proteins in Candida albicans because of their localization and their function, even if more than half of them have no characterized homolog in the databases. In this study, we focused on the IFF protein family, investigating their exposure on the cell surface and the sequences that determine their subcellular localization. Protein localization and surface exposure were monitored by the addition of a V5 tag on all members of the family. The data obtained using the complete proteins showed for Iff3 (or -9), Iff5, Iff6, and Iff8 a covalent linkage to the β-1,6-glucan network but, remarkably, showed that Iff2/Hyr3 was linked through disulfide bridges or NaOH-labile bonds. However, since some proteins of the Iff family were undetectable, we designed chimeric constructions using the last 60 amino acids of these proteins to test the localization signal. These constructions showed a β-1,6-glucan linkage for Iff1/Rbr3, Iff2/Hyr3, Iff4 and Iff7/Hyr4 C-terminal-Iff5 fusion proteins, and a membrane localization for the Iff10/Flo9 C terminus-Iff5 fusion protein. Immunofluorescence analyses coupled to these cell fraction data confirmed the importance of the length of the central serine/threonine-rich region for cell surface exposure. Further analysis of the Iff2/Hyr3 linkage to the cell surface showed for the first time that a serine/threonine central region of a GPI-anchored protein may be responsible for the disulfide and the NaOH bonds to the glucan and glycoproteins network and may also override the signal of the proximal ω site region.
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