ArticleApplied and environmental microbiology2011
Toward improvement of erythromycin A production in an industrial Saccharopolyspora erythraea strain via facilitation of genetic manipulation with an artificial attB site for specific recombination.
Article in Applied and environmental microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 41 citations in OpenAlex.
- Evaluation of vector system in Saccharopolyspora erythraea and construction of new replicative vector.Applied microbiology and biotechnology · 2026Article
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- Perspectives on Genetically Engineered Microorganisms and Their Regulation in the United States.ACS synthetic biology · 2024Review
- Succession of endophytic bacterial community and its contribution to cinnamon oil production during cinnamon shade-drying process.Food chemistry. Molecular sciences · 2022Article
- PhoP- and GlnR-mediated regulation of metK transcription and its impact upon S-adenosyl-methionine biosynthesis in Saccharopolyspora erythraea.Microbial cell factories · 2022Article
- EngineeringJournal of fungi (Basel, Switzerland) · 2021Article
- Effect of Castor Oil on Bioprocess Parameters of Erythromycin Fermentation byIranian journal of biotechnology · 2021Article
- Genomic Sequence Analysis of Methicillin- and Carbapenem-Resistant Bacteria Isolated from Raw Sewage.Microbiology spectrum · 2021Article
- Comparative Transcriptome Analysis Demonstrates the Positive Effect of the Cyclic AMP Receptor Protein Crp on Daptomycin Biosynthesis inFrontiers in bioengineering and biotechnology · 2021Article
- Engineering the Erythromycin-Producing StrainFrontiers in microbiology · 2020Article
- Recent advances in the discovery and combinatorial biosynthesis of microbial 14-membered macrolides and macrolactones.Journal of industrial microbiology & biotechnology · 2019Review
- Enhanced lincomycin production by co-overexpression of metK1 and metK2 in Streptomyces lincolnensis.Journal of industrial microbiology & biotechnology · 2018Article
- Article
- SACE_0012, a TetR-family transcriptional regulator, affects the morphogenesis of Saccharopolyspora erythraea.Current microbiology · 2013Article
- Saccharopolyspora erythraea's genome is organised in high-order transcriptional regions mediated by targeted degradation at the metabolic switch.BMC genomics · 2013Article
- Improvement of FK506 production in Streptomyces tsukubaensis by genetic enhancement of the supply of unusual polyketide extender units via utilization of two distinct site-specific recombination systems.Applied and environmental microbiology · 2012Article
- An erythromycin process improvement using the diethyl methylmalonate-responsive (Dmr) phenotype of the Saccharopolyspora erythraea mutB strain.Applied microbiology and biotechnology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Large-scale production of erythromycin A (Er-A) relies on the organism Saccharopolyspora erythraea, in which lack of a typical attB site largely impedes the application of phage ΦC31 integrase-mediated recombination into site-specific engineering. We herein report construction of an artificial attB site in an industrial S. erythraea strain, HL3168 E3, in an effort to break the bottleneck previously encountered during genetic manipulation mainly from homologous or unpredictable nonspecific integration. Replacement of a cryptic gene, nrps1-1, with a cassette containing eight attB DNA sequences did not affect the high Er-producing ability, setting the stage for precisely engineering the industrial Er-producing strain for foreign DNA introduction with a reliable conjugation frequency. Transfer of either exogenous or endogenous genes of importance to Er-A biosynthesis, including the S-adenosylmethionine synthetase gene for positive regulation, vhb for increasing the oxygen supply, and two tailoring genes, eryK and eryG, for optimizing the biotransformation at the late stage, was achieved by taking advantage of this facility, allowing systematic improvement of Er-A production as well as elimination of the by-products Er-B and Er-C in fermentation. The strategy developed here can generally be applicable to other strains that lack the attB site.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.