ArticleJournal of bacteriology2011
The biofilm inhibitor carolacton disturbs membrane integrity and cell division of Streptococcus mutans through the serine/threonine protein kinase PknB.
Article in Journal of bacteriology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 1 synthesis or guideline pooled it, 71 citations in OpenAlex.
- Stool metatranscriptomics: A technical guideline for mRNA stabilisation and isolation.BMC genomics · 2015Guideline
- Treatment of biofilms in bacterial vaginosis by an amphoteric tenside pessary-clinical study and microbiota analysis.Microbiome · 2017Trial
- Computational and preliminary screening of anti-caries active compounds of Caesalpinia sappan.Scientific reports · 2026Article
- In Situ Biofilm Affinity-Based Protein Profiling Identifies the Streptococcal Hydrolase GbpB as the Target of a Carolacton-Inspired Chemical Probe.Journal of the American Chemical Society · 2024Article
- Collaboration in Natural Product Total Synthesis: Carolacton - A Decade of Discovery.Synlett : accounts and rapid communications in synthetic organic chemistry · 2021Article
- Article
- Natural products as inspiration for the development of bacterial antibiofilm agents.Natural product reports · 2020Review
- Instructive Advances in Chemical Microbiology Inspired by Nature's Diverse Inventory of Molecules.ACS infectious diseases · 2020Review
- The Usefulness of Microalgae Compounds for Preventing Biofilm Infections.Antibiotics (Basel, Switzerland) · 2019Review
- Effects of Complex DNA and MVs with GTF Extracted fromMolecules (Basel, Switzerland) · 2019Article
- Synthetic Simplification of Carolacton Enables Chemical Genetic Studies inACS infectious diseases · 2019Article
- TargetingMedChemComm · 2019Review
- The inhibitory effects of polypyrrole on the biofilm formation of Streptococcus mutans.PloS one · 2019Article
- Deep sequencing of biofilm microbiomes on dental composite materials.Journal of oral microbiology · 2019Article
- Remodeling of the Streptococcus mutans proteome in response to LrgAB and external stresses.Scientific reports · 2017Article
- Natural Products as Platforms To Overcome Antibiotic Resistance.Chemical reviews · 2017Review
- Diverted Total Synthesis of Carolacton-Inspired Analogs Yields Three Distinct Phenotypes in Streptococcus mutans Biofilms.Journal of the American Chemical Society · 2017Article
- Ser/Thr protein kinase PrkC-mediated regulation of GroEL is critical for biofilm formation inNPJ biofilms and microbiomes · 2017Article
- The biofilm inhibitor Carolacton inhibits planktonic growth of virulent pneumococci via a conserved target.Scientific reports · 2016Article
- Carolacton Treatment Causes Delocalization of the Cell Division Proteins PknB and DivIVa in Streptococcus mutans in vivo.Frontiers in microbiology · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Carolacton, a secondary metabolite isolated from the myxobacterium Sorangium cellulosum, disturbs Streptococcus mutans biofilm viability at nanomolar concentrations. Here we show that carolacton causes leakage of cytoplasmic content (DNA and proteins) in growing cells at low pH and provide quantitative data on the membrane damage. Furthermore, we demonstrate that the biofilm-specific activity of carolacton is due to the strong acidification occurring during biofilm growth. The chemical conversion of the ketocarbonic function of the molecule to a carolacton methylester did not impact its activity, indicating that carolacton is not functionally activated at low pH by a change of its net charge. A comparative time series microarray analysis identified the VicKRX and ComDE two-component signal transduction systems and genes involved in cell wall metabolism as playing essential roles in the response to carolacton treatment. A sensitivity testing of mutants with deletions of all 13 viable histidine kinases and the serine/threonine protein kinase PknB of S. mutans identified only the ΔpknB deletion mutant as being insensitive to carolacton treatment. A strong overlap between the regulon of PknB in S. mutans and the genes affected by carolacton treatment was found. The data suggest that carolacton acts by interfering with PknB-mediated signaling in growing cells. The resulting altered cell wall morphology causes membrane damage and cell death at low pH.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.