ArticleMethods (San Diego, Calif.)2011
Simple screening method for improving membrane protein thermostability.
Article in Methods (San Diego, Calif.), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Biophysical Characterization of Membrane Proteins.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Fluorescence-Detection Size-Exclusion Chromatography-Based Thermostability Assay for Membrane Proteins.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Lipid Membrane Mimetics in Functional and Structural Studies of Integral Membrane Proteins.Membranes · 2021Review
- Article
- Fluorescence-detection size-exclusion chromatography utilizing nanobody technology for expression screening of membrane proteins.Communications biology · 2021Article
- Article
- Review
- Single-Particle Cryo-EM of Membrane Proteins.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Structure Determination of Membrane Proteins Using X-Ray Crystallography.Methods in molecular biology (Clifton, N.J.) · 2021Article
- An improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification.Communications biology · 2020Article
- Article
- Cryo-electron microscopy structure and potential enzymatic function of human six-transmembrane epithelial antigen of the prostate 1 (STEAP1).The Journal of biological chemistry · 2020Article
- ATP-dependent thermostabilization of human P-glycoprotein (ABCB1) is blocked by modulators.The Biochemical journal · 2019Article
- High-throughput stability screening for detergent-solubilized membrane proteins.Scientific reports · 2019Article
- Cryo-EM structures of human STEAP4 reveal mechanism of iron(III) reduction.Nature communications · 2018Article
- Effects of Mutations and Ligands on the Thermostability of the l-Arginine/Agmatine Antiporter AdiC and Deduced Insights into Ligand-Binding of Human l-Type Amino Acid Transporters.International journal of molecular sciences · 2018Article
- Expression and purification of the mammalian translocator protein for structural studies.PloS one · 2018Article
- Membrane protein structure determination by SAD, SIR, or SIRAS phasing in serial femtosecond crystallography using an iododetergent.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Structure of the STRA6 receptor for retinol uptake.Science (New York, N.Y.) · 2016Article
- An Aromatic Cap Seals the Substrate Binding Site in an ECF-Type S Subunit for Riboflavin.Journal of molecular biology · 2016Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
Biochemical and biophysical analysis on integral membrane proteins often requires monodisperse and stable protein samples. Here we describe a method to characterize protein thermostability by measuring its melting temperature in detergent using analytical size-exclusion chromatography. This quantitative method can be used to screen for compounds and conditions that stabilize the protein. With this technique we were able to assess and improve the thermostability of several membrane proteins. These conditions were in turn used to assist purification, to identify protein ligand and to improve crystal quality.
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