ArticleJournal of proteomics2011
2-D DIGE uterine endothelial proteomic profile for maternal chronic binge-like alcohol exposure.
Article in Journal of proteomics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
12 citing papers in PubMed, 17 citations in OpenAlex.
- Article
- Early Abnormal Placentation and Evidence of Vascular Endothelial Growth Factor System Dysregulation at the Feto-Maternal Interface After Periconceptional Alcohol Consumption.Frontiers in physiology · 2021Review
- Gestational binge alcohol-induced alterations in maternal uterine artery transcriptome.Reproductive toxicology (Elmsford, N.Y.) · 2019Article
- Fetal regional brain protein signature in FASD rat model.Reproductive toxicology (Elmsford, N.Y.) · 2018Article
- Chronic binge alcohol consumption during pregnancy alters rat maternal uterine artery pressure response.Alcohol (Fayetteville, N.Y.) · 2016Article
- Effects of L-glutamine supplementation on maternal and fetal hemodynamics in gestating ewes exposed to alcohol.Amino acids · 2014Article
- Chronic binge alcohol exposure during pregnancy impairs rat maternal uterine vascular function.Alcoholism, clinical and experimental research · 2014Article
- Interactive effects of in vitro binge-like alcohol and ATP on umbilical endothelial nitric oxide synthase post-translational modifications and redox modulation.Reproductive toxicology (Elmsford, N.Y.) · 2014Article
- Recent advances in quantitative neuroproteomics.Methods (San Diego, Calif.) · 2013Review
- Alcohol-induced alterations in maternal uterine endothelial proteome: a quantitative iTRAQ mass spectrometric approach.Reproductive toxicology (Elmsford, N.Y.) · 2012Article
- Vascular effects of maternal alcohol consumption.American journal of physiology. Heart and circulatory physiology · 2012Review
- Multiplexed digital quantification of binge-like alcohol-mediated alterations in maternal uterine angiogenic mRNA transcriptome.Physiological genomics · 2012Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Little is known about alcohol effects on the utero-placental compartment during pregnancy. For the first time, we utilized 2-D DIGE quantitative proteomics to evaluate the role of the uterus in Fetal Alcohol Spectrum Disorders (FASD) pathogenesis. Uterine artery endothelial cells were isolated from pregnant ewes, FAC sorted, validated, and maintained in culture. To mimic maternal binge drinking patterns, cells were cultured in the absence or presence of alcohol (300 mg/dl) in a compensating sealed humidified chamber system equilibrated with aqueous alcohol for 3 h on 3 consecutive days for two weeks. CyDye switch combined with 2-D DIGE followed by MALDI-TOF and tandem MS/MS were utilized. Validation was performed using Western immunoblot analysis. Chronic binge-like alcohol significantly (P<0.05) decreased 30 proteins and increased 19 others. Gene-enrichment and functional annotation cluster analysis revealed significant enrichment (P<0.05) in three categories: glutathione S transferase, thioredoxin, and vesicle transport-related. Furthermore, alcohol differentially altered proteins with certain isoforms being downregulated while others were upregulated. In summary, binge alcohol has specific effects on the maternal uterine proteome, especially those related to oxidative stress. The current study also demonstrates a great need to utilize proteomic approaches for diagnostic, mechanistic and therapeutic aspects of FASD.
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Registered trials
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