ArticleBMC biotechnology2011
Characterization of mercury bioremediation by transgenic bacteria expressing metallothionein and polyphosphate kinase.
Article in BMC biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 102 citations in OpenAlex.
- Sustainable production of myoglobin meat protein in plant chloroplasts.Frontiers in plant science · 2026Article
- Microbial Contributions to Heavy Metal Phytoremediation in Agricultural Soils: A Review.Microorganisms · 2024Review
- Unraveling the multifaceted resilience of arsenic resistant bacteriumFrontiers in microbiology · 2023Article
- The Microbiology of Metal Mine Waste: Bioremediation Applications and Implications for Planetary Health.GeoHealth · 2021Review
- An implication of biotransformation in detoxification of mercury contamination by Morganella sp. strain IITISM23.Environmental science and pollution research international · 2021Article
- Soil Acidobacteria Strain AB23 Resistance to Oxidative Stress Through Production of Carotenoids.Microbial ecology · 2021Article
- Bioremediation of toxic heavy metals (THMs) contaminated sites: concepts, applications and challenges.Environmental science and pollution research international · 2020Review
- Polyphosphate FunctionsmBio · 2020Article
- Microalgal Metallothioneins and Phytochelatins and Their Potential Use in Bioremediation.Frontiers in microbiology · 2020Review
- Expression of metallothionein encoding gene bmtA in biofilm-forming marine bacterium Pseudomonas aeruginosa N6P6 and understanding its involvement in Pb(II) resistance and bioremediation.Environmental science and pollution research international · 2019Article
- Bioaccumulation of Arsenic by Engineered Escherichia coli Cells Expressing Rice Metallothionein Isoforms.Current microbiology · 2018Article
- Inorganic polyphosphates and heavy metal resistance in microorganisms.World journal of microbiology & biotechnology · 2018Review
- In a quest for engineering acidophiles for biomining applications: challenges and opportunities.Genes · 2018Review
- Polyphosphate inFrontiers in microbiology · 2018Article
- Heavy Metal Removal by Bioaccumulation Using Genetically Engineered Microorganisms.Frontiers in bioengineering and biotechnology · 2018Review
- Diversity, community structure, and bioremediation potential of mercury-resistant marine bacteria of estuarine and coastal environments of Odisha, India.Environmental science and pollution research international · 2016Article
- Toward Bioremediation of Methylmercury Using Silica Encapsulated Escherichia coli Harboring the mer Operon.PloS one · 2016Article
- Oxidative stress protection by polyphosphate--new roles for an old player.Current opinion in microbiology · 2015Review
- Accumulation of polyphosphate in Lactobacillus spp. and its involvement in stress resistance.Applied and environmental microbiology · 2014Article
- "NiCo Buster": engineering E. coli for fast and efficient capture of cobalt and nickel.Journal of biological engineering · 2014Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe use of transgenic bacteria has been proposed as a suitable alternative for mercury remediation. Ideally, mercury would be sequestered by metal-scavenging agents inside transgenic bacteria for subsequent retrieval. So far, this approach has produced limited protection and accumulation. We report here the development of a transgenic system that effectively expresses metallothionein (mt-1) and polyphosphate kinase (ppk) genes in bacteria in order to provide high mercury resistance and accumulation.
resultsIn this study, bacterial transformation with transcriptional and translational enhanced vectors designed for the expression of metallothionein and polyphosphate kinase provided high transgene transcript levels independent of the gene being expressed. Expression of polyphosphate kinase and metallothionein in transgenic bacteria provided high resistance to mercury, up to 80 μM and 120 μM, respectively. Here we show for the first time that metallothionein can be efficiently expressed in bacteria without being fused to a carrier protein to enhance mercury bioremediation. Cold vapor atomic absorption spectrometry analyzes revealed that the mt-1 transgenic bacteria accumulated up to 100.2 ± 17.6 μM of mercury from media containing 120 μM Hg. The extent of mercury remediation was such that the contaminated media remediated by the mt-1 transgenic bacteria supported the growth of untransformed bacteria. Cell aggregation, precipitation and color changes were visually observed in mt-1 and ppk transgenic bacteria when these cells were grown in high mercury concentrations.
conclusionThe transgenic bacterial system described in this study presents a viable technology for mercury bioremediation from liquid matrices because it provides high mercury resistance and accumulation while inhibiting elemental mercury volatilization. This is the first report that shows that metallothionein expression provides mercury resistance and accumulation in recombinant bacteria. The high accumulation of mercury in the transgenic cells could present the possibility of retrieving the accumulated mercury for further industrial applications.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.