ArticleChemical research in toxicology2011
Identification of potential protein targets of isothiocyanates by proteomics.
Article in Chemical research in toxicology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed, 73 citations in OpenAlex.
- Isothiocyanates in Cancer Therapeutics - A Molecular Perspective.Chemistry & biodiversity · 2026Review
- Direct inhibition of the TXNIP-NLRP3-GSDMD pathway reduces pyroptosis in colonocytes and alleviates ulcerative colitis in mice by the small compound PEITC.Acta pharmacologica Sinica · 2025Article
- Multiple glutathione-S-transferases detoxify diverse glucosinolate-based defenses of Brassicales plants in a generalist lepidopteran herbivore (Spodoptera littoralis).Communications biology · 2025Article
- Quantitative chemical proteomics reveals that phenethyl isothiocyanate covalently targets BID to promote apoptosis.Cell death discovery · 2024Article
- The Antimicrobial Effects of Myrosinase Hydrolysis Products Derived from Glucosinolates Isolated fromPlants (Basel, Switzerland) · 2024Article
- Isothiocyanates as Tubulin Polymerization Inhibitors-Synthesis and Structure-Activity Relationship Studies.International journal of molecular sciences · 2023Article
- The Importance of Microorganisms for Sustainable Agriculture-A Review.Metabolites · 2022Review
- Covalent Modification of Proteins by Plant-Derived Natural Products: Proteomic Approaches and Biological Impacts.Proteomics · 2021Review
- Sulforaphane: Expected to Become a Novel Antitumor Compound.Oncology research · 2020Review
- Prospective evaluation of the lymph node proteome in dogs with multicentric lymphoma supplemented with sulforaphane.Journal of veterinary internal medicine · 2020Article
- Mechanism of selective anticancer activity of isothiocyanates relies on differences in DNA damage repair between cancer and healthy cells.European journal of nutrition · 2020Article
- Article
- Various modes of action of dietary phytochemicals, sulforaphane and phenethyl isothiocyanate, on pathogenic bacteria.Scientific reports · 2019Article
- Yeast screening system reveals the inhibitory mechanism of cancer cell proliferation by benzyl isothiocyanate through down-regulation of Mis12.Scientific reports · 2019Article
- Targeting allosteric site of AKT by 5,7-dimethoxy-1,4-phenanthrenequinone suppresses neutrophilic inflammation.EBioMedicine · 2019Article
- Bioinformatics Analysis of Potential Key Genes in Trastuzumab-Resistant Gastric Cancer.Disease markers · 2019Article
- Phenethyl Isothiocyanate, a Dual Activator of Transcription Factors NRF2 and HSF1.Molecular nutrition & food research · 2018Review
- Physiological relevance of covalent protein modification by dietary isothiocyanates.Journal of clinical biochemistry and nutrition · 2018Review
- Functional foods and their role in cancer prevention and health promotion: a comprehensive review.American journal of cancer research · 2017Review
- Isothiocyanates as effective agents against enterohemorrhagic Escherichia coli: insight to the mode of action.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
Abstract
Isothiocyanates (ITCs), such as phenethyl isothiocyanate (PEITC) and sulforaphane (SFN), are effective cancer chemopreventive compounds. It is believed that the major mechanism for the cancer preventive activity of ITCs is through the induction of cell cycle arrest and apoptosis. However, the upstream molecular targets of ITCs have been underexplored until recently. To identify proteins that are covalently modified by ITCs, human non-small cell lung cancer A549 cells were treated with (14)C-PEITC and (14)C-SFN, and the cell lysates were extracted for analysis by 2-D gel electrophoresis and mass spectrometry. After superimposing the colloidal Coomassie blue protein staining pattern with the pattern of radioactivity obtained from X-ray films, it was clear that only a small fraction of cellular proteins contained radioactivity, presumably resulting from selective binding with PEITC or SFN via thiocarbamation. More than 30 proteins with a variety of biological functions were identified with high confidence. Here, we report the identities of these potential ITC target proteins and discuss their biological relevance. The discovery of the protein targets may facilitate studies of the mechanisms by which ITCs exert their cancer preventive activity and provide the molecular basis for designing more efficacious ITC compounds.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.