ArticleBiotechnology and bioengineering2012
Heparan sulfate proteoglycan mediates shear stress-induced endothelial gene expression in mouse embryonic stem cell-derived endothelial cells.
Article in Biotechnology and bioengineering, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 64 citations in OpenAlex.
- Mechanosensing by Vascular Endothelium.Annual review of physiology · 2024Review
- GAN-WGCNA: Calculating gene modules to identify key intermediate regulators in cocaine addiction.PloS one · 2024Article
- Vascular mechanotransduction.Physiological reviews · 2023Review
- Spatio-temporal dynamics enhance cellular diversity, neuronal function and further maturation of human cerebral organoids.Communications biology · 2023Article
- Biomechanical, biophysical and biochemical modulators of cytoskeletal remodelling and emergent stem cell lineage commitment.Communications biology · 2023Review
- Sulodexide improves vascular permeability via glycocalyx remodelling in endothelial cells during sepsis.Frontiers in immunology · 2023Article
- Glycocalyx heparan sulfate cleavage promotes endothelial cell angiopoietin-2 expression by impairing shear stress-related AMPK/FoxO1 signaling.JCI insight · 2022Article
- Effect of high glucose on glycosaminoglycans in cultured retinal endothelial cells and rat retina.Glycobiology · 2022Article
- The glycocalyx: a central regulator of vascular function.American journal of physiology. Regulatory, integrative and comparative physiology · 2021Review
- Alveolar epithelial glycocalyx shedding aggravates the epithelial barrier and disrupts epithelial tight junctions in acute respiratory distress syndrome.Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie · 2021Article
- Correlation Between Wall Shear Stress and Acute Degradation of the Endothelial Glycocalyx During Cardiopulmonary Bypass.Journal of cardiovascular translational research · 2020Article
- A sticky proposition: The endothelial glycocalyx and von Willebrand factor.Journal of thrombosis and haemostasis : JTH · 2020Article
- Empagliflozin restores the integrity of the endothelial glycocalyx in vitro.Molecular and cellular biochemistry · 2019Article
- Pro-atherosclerotic disturbed flow disrupts caveolin-1 expression, localization, and function via glycocalyx degradation.Journal of translational medicine · 2018Article
- Regulation of eosinophil recruitment and allergic airway inflammation by heparan sulfate proteoglycan (HSPG) modifying enzymes.Experimental lung research · 2018Article
- Fluid shear stress induces upregulation of COX-2 and PGIAmerican journal of physiology. Heart and circulatory physiology · 2017Article
- Pharmacokinetic profile that reduces nephrotoxicity of gentamicin in a perfused kidney-on-a-chip.Biofabrication · 2016Article
- Loss of function in heparan sulfate elongation genes EXT1 and EXT 2 results in improved nitric oxide bioavailability and endothelial function.Journal of the American Heart Association · 2014Article
- Sox18 preserves the pulmonary endothelial barrier under conditions of increased shear stress.Journal of cellular physiology · 2014Article
- Role of extracellular matrix signaling cues in modulating cell fate commitment for cardiovascular tissue engineering.Advanced healthcare materials · 2014Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
It has been shown that shear stress plays a critical role in promoting endothelial cell (EC) differentiation from embryonic stem cell (ESC)-derived ECs. However, the underlying mechanisms mediating shear stress effects in this process have yet to be investigated. It has been reported that the glycocalyx component heparan sulfate proteoglycan (HSPG) mediates shear stress mechanotransduction in mature EC. In this study, we investigated whether cell surface HSPG plays a role in shear stress modulation of EC phenotype. ESC-derived EC were subjected to shear stress (5 dyn/cm(2)) for 8 h with or without heparinase III (Hep III) that digests heparan sulfate. Immunostaining showed that ESC-derived EC surfaces contain abundant HSPG, which could be cleaved by Hep III. We observed that shear stress significantly increased the expression of vascular EC-specific marker genes (vWF, VE-cadherin, PECAM-1). The effect of shear stress on expression of tight junction protein genes (ZO-1, OCLD, CLD5) was also evaluated. Shear stress increased the expression of ZO-1 and CLD5, while it did not alter the expression of OCLD. Shear stress increased expression of vasodilatory genes (eNOS, COX-2), while it decreased the expression of the vasoconstrictive gene ET1. After reduction of HSPG with Hep III, the shear stress-induced expression of vWF, VE-cadherin, ZO-1, eNOS, and COX-2, were abolished, suggesting that shear stress-induced expression of these genes depends on HSPG. These findings indicate for the first time that HSPG is a mechanosensor mediating shear stress-induced EC differentiation from ESC-derived EC cells.
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