Increase in Drosophila melanogaster longevity due to rasayana diet: Preliminary results.

We report preliminary results from an ongoing series of experiments on lifespan extension by appropriately modified Ayurvedic rasayanas in animal models. Here data are presented indicating lifespan extensions of 51-55% (up to 70-95% in the pilot experiment) in a standard strain of Drosophila melanogaster (Oregon-K) using a standard rasayana (Ayurvedic herbal formulation for life-extension) suitably adapted for insects. In a first experiment, two groups of 20 unmated D. melanogaster strain Oregon-K kept at 22°C received either rasayana or standard yeast diet; days of death were recorded. Another experiment investigated possible sex differences; equal sized (N = 30) groups of similar males, females, and controls were compared. Life lengths of all controls were in the strain's usual range: in Experiment 1, control life lengths were minimum 40 to maximum 53 days; experimental group figures were 81-91 days; groups were completely separated, experimental group minimum life length being 28 days more than control group maximum life length, i.e., about 2.5 full distribution widths - a sign test for the null hypothesis yields p < 2(-20), i.e., 10(-6) as maximum p. Experiment 2 found no differences between life lengths of males and females; but the maximum life length of 30 controls (60 days in males and 66 days in females) was once again far shorter than the minimum life length of the 60 in the two experimental groups, strengthening the findings of Experiment 1. Despite group sizes being relatively small, results are conclusive: the rasayanas in question increase D. melanogaster strain Oregon-K life length. The complexity of the formulation suggests that multiple mechanisms are involved - worth further investigation.