ArticleEye (London, England)2011
Optimizing evaluation of Lissamine Green parameters for ocular surface staining.
Article in Eye (London, England), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Dry eye in the upper blepharoplasty patient: a study comparing orbicularis-sparing versus orbicularis-excising techniques.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2023Trial
- Article
- Corneal Fluorescein Staining in FDA-Reviewed Dry Eye Registrational Clinical Trials: Evidence of Limited Endpoint Responsiveness.Ophthalmic research · 2026Article
- Lissamine Green in Ophthalmology: A Comprehensive Review of Diagnostic and Clinical Applications.Ophthalmology and therapy · 2025Review
- A Comparison of Physical Characteristics in Different Brands and Staining Techniques in a Brand of Lissamine Green Strips.Journal of clinical medicine · 2025Article
- Effect of lid-wiper epitheliopathy on the corneal epithelial thickness distribution: a novel semiautomatic quantitative method.BMC ophthalmology · 2025Article
- Overview of Dry Eye Disease for Primary Care Physicians.Medicina (Kaunas, Lithuania) · 2025Review
- Comparison of manual versus automated thermal lid therapy with expression for meibomian gland dysfunction in patients with dry eye disease.Scientific reports · 2024Article
- Compatibility of a New Ocular Surface Dye with Disposable and Bi-Weekly Soft Contact Lenses: An Experimental Study.Life (Basel, Switzerland) · 2024Article
- Prevalence and Risk Factors of Dry Eye Disease in the South of Palestine.The Malaysian journal of medical sciences : MJMS · 2024Article
- Ocular surface staining: Current concepts and techniques.Indian journal of ophthalmology · 2023Review
- Small-extracellular vesicles and their microRNA cargo from porcine follicular fluids: the potential association with oocyte quality.Journal of animal science and biotechnology · 2022Article
- Canonical Grading Scales of Corneal and Conjunctival Staining Based on Psychophysical and Physical Attributes.Translational vision science & technology · 2021Article
- The HYLAN M Study: Efficacy of 0.15% High Molecular Weight Hyaluronan Fluid in the Treatment of Severe Dry Eye Disease in a Multicenter Randomized Trial.Journal of clinical medicine · 2020Article
- Impact of Improper Approach to Identify Lid Wiper Epitheliopathy (LWE).Clinical ophthalmology (Auckland, N.Z.) · 2020Article
- Preclinical Efficacy Comparison of Cyclosporine Ophthalmic Solution 0.09% vs Cyclosporine Ophthalmic Emulsion 0.05% vs Ciclosporin Ophthalmic Emulsion 0.1% in a NOD Mouse Model of Dry Eye Disease.Clinical ophthalmology (Auckland, N.Z.) · 2020Article
- An in vitro depth of injury prediction model for a histopathologic classification of EPA and GHS eye irritants.Toxicology in vitro : an international journal published in association with BIBRA · 2019Article
- Presence of Histatin-1 in Human Tears and Association with Aqueous Deficient Dry Eye Diagnosis: A Preliminary Study.Scientific reports · 2019Article
- Current Approach to Dry Eye Disease.Clinical reviews in allergy & immunology · 2015Review
- Diagnosis of dry eye disease and emerging technologies.Clinical ophthalmology (Auckland, N.Z.) · 2014Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
purposeThe recently published seminal dry eye workshop proceedings defined Lissamine Green (LG), an organic dye, as a gold standard for demonstrating ocular surface staining. The purpose of the current study was to determine the optimal parameters of 1% LG instillation for the ocular surface examination in dry eye patients.
designProspective and observational quality improvement study.
methodsA quality improvement study evaluated 16 eyes from eight dry eye patients with different levels of severity. LG (1%), in three volumes (5, 10, and 20 μl) was instilled into the conjunctival cul-de-sac, and four masked observers with different levels of clinical expertise examined the patients with and without red filter. The staining pattern of the conjunctiva and cornea was documented with the Oxford scale within 4 min of LG instillation. Optimal volume and inter-observer reliability were assessed.
resultsAll dye volumes were tolerated well by all patients. Experienced observers preferred 10 μl volume because of the ease of examination and accuracy. Although instillation of 20 μl yielded similar scores as 10 μl, it resulted in overflow of the lid and facial skin staining. The use of red filter significantly improved reading scores (P<0.01). Inter-observer reliability was higher for conjunctival scores than for corneal scores for all patients. The highest reliability was demonstrated with 10 μl volume and increased with greater experience of the observer.
conclusionsOcular surface examination with instillation of 10 μl 1% LG has good inter-observer reliability and is well tolerated. Observation through a red filter facilitates the examination.
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