ArticleGlycobiology2012
Directing the biological activities of heparan sulfate oligosaccharides using a chemoenzymatic approach.
Article in Glycobiology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 23 citations in OpenAlex.
- Targeting heparan sulfate-protein interactions with oligosaccharides and monoclonal antibodies.Frontiers in molecular biosciences · 2023Review
- Construction of heparan sulfate microarray for investigating the binding of specific saccharide sequences to proteins.Glycobiology · 2021Article
- 3-O sulfation of heparin leads to hepatotropism and longer circulatory half-life.Thrombosis research · 2018Article
- Brain heparan sulphate proteoglycans are altered in developing foetus when exposed to in-utero hyperglycaemia.Metabolic brain disease · 2017Article
- The Good the Bad and the Ugly of Glycosaminoglycans in Tissue Engineering Applications.Pharmaceuticals (Basel, Switzerland) · 2017Review
- New Applications of Heparin and Other Glycosaminoglycans.Molecules (Basel, Switzerland) · 2017Review
- Obstacles and solutions for chemical synthesis of syndecan-3 (53-62) glycopeptides with two heparan sulfate chains.Carbohydrate research · 2016Article
- Divergent Synthesis of Heparan Sulfate Oligosaccharides.The Journal of organic chemistry · 2015Article
- Fell-Muir Lecture: Heparan sulphate and the art of cell regulation: a polymer chain conducts the protein orchestra.International journal of experimental pathology · 2015Article
- Can we produce heparin/heparan sulfate biomimetics using "mother-nature" as the gold standard?Molecules (Basel, Switzerland) · 2015Review
- Capillary electrophoresis for total glycosaminoglycan analysis.Analytical and bioanalytical chemistry · 2014Article
- Chemical synthesis of a heparan sulfate glycopeptide: syndecan-1.Angewandte Chemie (International ed. in English) · 2012Article
- Uncovering biphasic catalytic mode of C5-epimerase in heparan sulfate biosynthesis.The Journal of biological chemistry · 2012Article
- Strategies in synthesis of heparin/heparan sulfate oligosaccharides: 2000-present.Advances in carbohydrate chemistry and biochemistry · 2012Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Heparan sulfate (HS) and heparin are highly sulfated polysaccharides exhibiting essential physiological functions. The sulfation patterns determine the functional selectivity for HS and heparin. Chemical synthesis of HS, especially those larger than a hexasaccharide, remains challenging. Enzymatic synthesis of HS has recently gained momentum. Here we describe the divergent assembly of HS heptasaccharides and nonasaccharides from a common hexasaccharide precursor. The hexasaccharide precursor was synthesized via a chemical method. The subsequent elongation, sulfation and epimerization were completed by glycosyltransferases, HS sulfotransferases and epimerase. Using the synthesized heptasaccharides, we discovered that the iduronic acid is critical for binding to fibroblast growth factor-2. We also designed a synthetic path to prepare a nonasaccharide with an antithrombin-binding affinity of 3 nM. Our method demonstrated the feasibility of combining chemical and enzymatic synthesis to prepare structurally defined HS oligosaccharides with desired biological activities.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.