ArticleBiomaterials2011
Directing human embryonic stem cell differentiation by non-viral delivery of siRNA in 3D culture.
Article in Biomaterials, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 47 citations in OpenAlex.
- Advancing the Landscape of RNAi Nanotherapeutics for Ischemic Heart Disease.Advanced materials (Deerfield Beach, Fla.) · 2026Review
- Important Aspects of siRNA Design for Optimal Efficacy In Vitro and In Vivo.International journal of cell biology · 2025Review
- Single cell transcriptomics reveals reduced stress response in stem cells manipulated using localized electric fields.Materials today. Bio · 2023Article
- The Delivery of RNA-Interference Therapies Based on Engineered Hydrogels for Bone Tissue Regeneration.Frontiers in bioengineering and biotechnology · 2020Review
- RNA-based scaffolds for bone regeneration: application and mechanisms of mRNA, miRNA and siRNA.Theranostics · 2020Review
- Nanomaterial-Based Approaches for Neural Regeneration.Pharmaceutics · 2019Review
- Direct transfection of clonal organoids in Matrigel microbeads: a promising approach toward organoid-based genetic screens.Nucleic acids research · 2018Article
- Optimized delivery of siRNA into 3D tumor spheroid cultures in situ.Scientific reports · 2018Article
- New Challenge: Mitochondrial Epigenetics?Stem cell reviews and reports · 2018Review
- Technologies for controlled, local delivery of siRNA.Journal of controlled release : official journal of the Controlled Release Society · 2015Review
- Application of biomaterials to advance induced pluripotent stem cell research and therapy.The EMBO journal · 2015Review
- Technology advancement for integrative stem cell analyses.Tissue engineering. Part B, Reviews · 2014Review
- Plasma membrane recovery kinetics of a microfluidic intracellular delivery platform.Integrative biology : quantitative biosciences from nano to macro · 2014Article
- Nanotopography-mediated reverse uptake for siRNA delivery into neural stem cells to enhance neuronal differentiation.Scientific reports · 2013Article
- Controlling mesenchymal stem cell gene expression using polymer-mediated delivery of siRNA.Biomacromolecules · 2012Article
- Combining topographical and genetic cues to promote neuronal fate specification in stem cells.Biomacromolecules · 2012Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Human embryonic stem cells (hESCs) hold great potential as a resource for regenerative medicine. Before achieving therapeutic relevancy, methods must be developed to control stem cell differentiation. It is clear that stem cells can respond to genetic signals, such as those imparted by nucleic acids, to promote lineage-specific differentiation. Here we have developed an efficient system for delivering siRNA to hESCs in a 3D culture matrix using lipid-like materials. We show that non-viral siRNA delivery in a 3D scaffolds can efficiently knockdown 90% of GFP expression in GFP-hESCs. We further show that this system can be used as a platform for directing hESC differentiation. Through siRNA silencing of the KDR receptor gene, we achieve concurrent downregulation (60-90%) in genes representative of the endoderm germ layer and significant upregulation of genes representative of the mesoderm germ layer (27-90 fold). This demonstrates that siRNA can direct stem cell differentiation by blocking genes representative of one germ layer and also provides a particularly powerful means to isolate the endoderm germ layer from the mesoderm and ectoderm. This ability to inhibit endoderm germ layer differentiation could allow for improved control over hESC differentiation to desired cell types.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.