ArticleJournal of neuroscience methods2011
Organotypic vibrosections: novel whole sagittal brain cultures.
Article in Journal of neuroscience methods, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- Ex vivo study of neuroinvasive and neurotropic viruses: what is current and what is next.FEMS microbiology reviews · 2025Review
- Cortical Organotypic Brain Slice Cultures to Examine Sex- and Age-Dependent Astrocyte-Mediated Synaptic Phagocytosis.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Investigating the use of cuprizone and lysolecithin to model demyelinationFrontiers in cellular neuroscience · 2025Article
- Melatonin Activates Anti-Inflammatory Features in Microglia in a Multicellular Context: Evidence from Organotypic Brain Slices and HMC3 Cells.Biomolecules · 2023Article
- Article
- Co-cultures of cerebellar slices from mice with differentF1000Research · 2022Article
- Evidence of Microglial Immune Response Following Coronavirus PHEV Infection of CNS.Frontiers in immunology · 2021Article
- Microcontact Printing of Cholinergic Neurons in Organotypic Brain Slices.Frontiers in neurology · 2021Article
- A Bridge BetweenNoro psikiyatri arsivi · 2020Review
- Modeling α-Synucleinopathy in Organotypic Brain Slice Culture with Preformed α-Synuclein Amyloid Fibrils.Journal of Parkinson's disease · 2020Article
- Organotypic brain slice cultures to model neurodegenerative proteinopathies.Molecular neurodegeneration · 2019Review
- Organs to Cells and Cells to Organoids: The Evolution ofFrontiers in cellular neuroscience · 2019Review
- Modelling glioblastoma tumour-host cell interactions using adult brain organotypic slice co-culture.Disease models & mechanisms · 2018Article
- Three-Dimensional Models of the Human Brain Development and Diseases.Advanced healthcare materials · 2018Review
- Ex Vivo Measurement of Electrically Evoked Dopamine Release in Zebrafish Whole Brain.ACS chemical neuroscience · 2017Article
- SGPL1 (sphingosine phosphate lyase 1) modulates neuronal autophagy via phosphatidylethanolamine production.Autophagy · 2017Article
- Effects of Gualou Guizhi Decoction Aqueous Extract on Axonal Regeneration in Organotypic Cortical Slice Culture after Oxygen-Glucose Deprivation.Evidence-based complementary and alternative medicine : eCAM · 2017Article
- Alpha-Smooth Muscle Actin mRNA and Protein Are Increased in Isolated Brain Vessel Extracts of Alzheimer Mice.Pharmacology · 2016Article
- Green-Fluorescent Protein(+) Astrocytes Attach to Beta-Amyloid Plaques in an Alzheimer Mouse Model and Are Sensitive for Clasmatodendrosis.Frontiers in aging neuroscience · 2016Article
- Organotypic brain slice cultures: A review.Neuroscience · 2015Review
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3 authors.
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Abstract
In vitro cell culture models are of enormous importance in neuroscience research and organotypic brain slices are found to be a potent model very close to the in vivo situation. Brain slices can be cultured as single slices or as co-slices. However, there is need to culture whole brain sections, containing the complex functional architecture. The aim of the present study was to develop and characterize whole brain sagittal slice cultures (200μm organotypic vibrosections) from postnatal day 8 rats. We show that sagittal vibrosections can be cultured for several weeks and they maintain survival of cholinergic and dopaminergic neurons, as well as a strong capillary network. Partly long-distance cortico-striatal and cortico-hippocampal nerve fibers were found using Mini-Ruby neurotracing. Dopaminergic nerve fibers extended from the mesencephalon, but in the striato-nigral tract and in the striatum only strong dense varicosities were found. The model also allows to study pathological triggers, such as e.g. hydrogen peroxide markedly increased propidiumiodide-positive nuclei in the hippocampus. In conclusion, our novel model provides an easy potent whole sagittal brain culture system that allows to study cholinergic and dopaminergic neurons together but also in close interaction with all other cells of the brain and with capillaries. It will be a great challenge in future to use this model to re-construct whole pathways. This vibrosection model may partly represent a close adult in vivo situation, which allows to study neurodegeneration and neuroprotection of cholinergic and dopaminergic neurons, which plays an important role in Alzheimer's and Parkinson's disease, respectively.
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