ArticleUrological research2012
Role of osteopontin in early phase of renal crystal formation: immunohistochemical and microstructural comparisons with osteopontin knock-out mice.
Article in Urological research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers, 1 of them a synthesis that pooled it.
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Who cites it
21 citing papers in PubMed, 1 synthesis or guideline pooled it, 40 citations in OpenAlex.
- Recent Progression and Treatment Approaches for the Kidney Stone Management.Current drug targets · 2025Pooled it
- Article
- Current Status of Protein Biomarkers in Urolithiasis-A Review of the Recent Literature.Journal of clinical medicine · 2023Review
- A New Parameter for Calcium Oxalate Stones: Impact of Linear Calculus Density on Non-Contrast Computed Tomography.Medicina (Kaunas, Lithuania) · 2023Article
- Osteopontin accumulates in basal deposits of human eyes with age-related macular degeneration and may serve as a biomarker of aging.Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc · 2022Article
- Hydroxycitrate prevents calcium oxalate crystallization and kidney injury in a nephrolithiasis rat model.Urolithiasis · 2022Article
- Article
- Mitochondrial Dysfunction and Kidney Stone Disease.Frontiers in physiology · 2020Review
- The Impact of Glycosylation of Osteopontin on Urinary Stone Formation.International journal of molecular sciences · 2019Article
- Inhibitive Effects of Huashi Pill on Formation of Renal Stones by Modulating Urine Biochemical Indexes and Osteopontin in Renal Stone Rat Models.Medical science monitor : international medical journal of experimental and clinical research · 2019Article
- Dynamic thiol/disulfide homeostasis as a novel indicator of oxidative stress in patients with urolithiasis.Investigative and clinical urology · 2019Article
- The effects of HAP and macrophage cells to the expression of inflammatory factors and apoptosis in HK-2 cells of vitro co-cultured system.Urolithiasis · 2018Article
- Long non-coding RNA CHCHD4P4 promotes epithelial-mesenchymal transition and inhibits cell proliferation in calcium oxalate-induced kidney damage.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2017Article
- Genome-Wide Gene Expression Profiling of Randall's Plaques in Calcium Oxalate Stone Formers.Journal of the American Society of Nephrology : JASN · 2017Article
- AGEs induce ectopic endochondral ossification in intervertebral discsEuropean cells & materials · 2016Article
- Osteopontin protects against high phosphate-induced nephrocalcinosis and vascular calcification.Kidney international · 2016Article
- Biomimetic Randall's plaque as an in vitro model system for studying the role of acidic biopolymers in idiopathic stone formation.Urolithiasis · 2015Article
- Osteopontin: A novel regulator at the cross roads of inflammation, obesity and diabetes.Molecular metabolism · 2014Review
- Multifunctional role of osteopontin in directing intrafibrillar mineralization of collagen and activation of osteoclasts.Acta biomaterialia · 2014Article
- Recurrent urolithiasis following parathyroidectomy for primary hyperparathyroidism.Annals of the Royal College of Surgeons of England · 2013Article
Corrections and comments
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Osteopontin (OPN) is an important matrix protein of renal calcium stone. However, the function of OPN in the early phase of renal crystal formation is not well defined. In this study, we examined OPN expression in the early phase of renal crystal formation with ultra-microstructural observations and immuno-TEM (transmission electron microscopy) in control and OPN knock-out (OPN-KO) mice. Glyoxylate (100 mg/kg) was intra-abdominally administered to male wild-type mice (C57BL/6, 8 weeks of age) and OPN-KO mice (C57BL/6, 8 weeks of age). Kidney was collected before and 6, 12, and 24 h after administration. We examined the relation between renal crystal formation and microstructural OPN location using TEM and immunohistochemical staining of OPN as well as western blotting and quantitative RT-PCR for OPN. OPN protein expression gradually increased in the renal cortex-medulla junction after glyoxylate administration, and OPN mRNA was increased until 12 h, but decreased at 24 h. In ultra-microstructural observation, OPN began to appear on the luminal side of renal distal tubular cells at 6 h and was gradually detected in the tubular lumen at 12 h. OPN was present in the crystal nuclei and collapsed mitochondria in the tubular lumen. In the OPN-KO mice, collapsed mitochondria were present, but no crystal nuclei formation were detected at 24 h. Based on the results this study proposed that the appearance of organelles, such as mitochondria and microvilli, in the tubular lumen after cell injury may be the starting point of crystal nucleus formation due to the aggregation ability of OPN.
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