ArticleMolecular biology reports2012
Molecular cloning of mevalonate pathway genes from Taraxacum brevicorniculatum and functional characterisation of the key enzyme 3-hydroxy-3-methylglutaryl-coenzyme A reductase.
Article in Molecular biology reports, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- The interaction networks of small rubber particle proteins in the latex ofFrontiers in plant science · 2024Article
- Molecular Genetic Research and Genetic Engineering ofPlants (Basel, Switzerland) · 2023Review
- Molecular cloning and characterization of Triterpenoid Biosynthetic Pathway Gene HMGS in Centella asiatica (Linn.).Molecular biology reports · 2022Article
- Metabolic and Transcriptomic Analyses Reveal the Effects of Ethephon onMolecules (Basel, Switzerland) · 2022Article
- Comparative proteome and metabolome analyses of latex-exuding and non-exuding Taraxacum koksaghyz roots provide insights into laticifer biology.Journal of experimental botany · 2020Article
- Natural rubber biosynthesis in plants, the rubber transferase complex, and metabolic engineering progress and prospects.Plant biotechnology journal · 2019Review
- The enzymes OSC1 and CYP716A263 produce a high variety of triterpenoids in the latex of Taraxacum koksaghyz.Scientific reports · 2019Article
- Fungal endophytes attune withanolide biosynthesis in Withania somnifera, prime to enhanced withanolide A content in leaves and roots.World journal of microbiology & biotechnology · 2019Article
- Comparative transcriptomics between high and low rubber producing Taraxacum kok-saghyz R. plants.BMC genomics · 2018Article
- Development of rubber-enriched dandelion varieties by metabolic engineering of the inulin pathway.Plant biotechnology journal · 2017Article
- Isoprenoid biosynthesis in dandelion latex is enhanced by the overexpression of three key enzymes involved in the mevalonate pathway.BMC plant biology · 2017Article
- Chloroplast genome resources and molecular markers differentiate rubber dandelion species from weedy relatives.BMC plant biology · 2017Article
- Article
- Functional diversity of genes for the biosynthesis of paeoniflorin and its derivatives in Paeonia.International journal of molecular sciences · 2013Article
- Laticifer-specific cis-prenyltransferase silencing affects the rubber, triterpene, and inulin content of Taraxacum brevicorniculatum.Plant physiology · 2012Article
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7 authors.
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Abstract
Taraxacum brevicorniculatum is known to produce high quality rubber. The biosynthesis of rubber is dependent on isopentenyl pyrophosphate (IPP) precursors derived from the mevalonate (MVA) pathway. The cDNA sequences of seven MVA pathway genes from latex of T. brevicorniculatum were isolated, including three cDNA sequences encoding for 3-hydroxy-3-methylglutaryl-coenzyme A (HMG-CoA) reductases (TbHMGR1-3). Expression analyses indicate an important role of TbHMGR1 as well as for the HMG-CoA synthase (TbHMGS), the diphosphomevalonate decarboxylase and the mevalonate kinase in the provision of precursors for rubber biosynthesis. The amino acid sequences of the TbHMGRs show the typical motifs described for plant HMGRs such as two transmembrane domains and a catalytic domain containing two HMG-CoA and two NADP(H) binding sites. The functionality of the HMGRs was demonstrated by complementation assay using an IPP auxotroph mutant of Escherichia coli. Furthermore, the transient expression of the catalytic domains of TbHMGR1 and TbHMGR2 in Nicotiana benthamiana resulted in a strong accumulation of sterol precursors, one of the major groups of pathway end-products.
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