ArticleFrontiers in microbiology2011
Growth of Acidithiobacillus Ferrooxidans ATCC 23270 in Thiosulfate Under Oxygen-Limiting Conditions Generates Extracellular Sulfur Globules by Means of a Secreted Tetrathionate Hydrolase.
Article in Frontiers in microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 38 citations in OpenAlex.
- Tetrathionate hydrolase from the acidophilic microorganisms.Frontiers in microbiology · 2024Review
- Physiological and Genomic Characterization of a Novel Obligately Chemolithoautotrophic, Sulfur-Oxidizing Bacterium of GenusMicroorganisms · 2023Article
- Review
- Genome sequencing and metabolic network reconstruction of a novel sulfur-oxidizing bacteriumFrontiers in microbiology · 2023Article
- Progress in bioleaching: fundamentals and mechanisms of microbial metal sulfide oxidation - part A.Applied microbiology and biotechnology · 2022Review
- Deep-SeamBio · 2022Article
- Reaction mechanism of tetrathionate hydrolysis based on the crystal structure of tetrathionate hydrolase from Acidithiobacillus ferrooxidans.Protein science : a publication of the Protein Society · 2021Article
- Microbially Influenced Corrosion of Stainless Steel by Acidithiobacillus ferrooxidans Supplemented with Pyrite: Importance of Thiosulfate.Applied and environmental microbiology · 2019Article
- Sulfur Oxidation in the Acidophilic AutotrophicFrontiers in microbiology · 2018Review
- Sulfur Metabolism ofFrontiers in microbiology · 2017Article
- Article
- Tetrathionate and Elemental Sulfur Shape the Isotope Composition of Sulfate in Acid Mine Drainage.Frontiers in microbiology · 2017Article
- Construction and characterization of tetH overexpression and knockout strains of Acidithiobacillus ferrooxidans.Journal of bacteriology · 2014Article
- Crystallization and preliminary X-ray diffraction analysis of tetrathionate hydrolase from Acidithiobacillus ferrooxidans.Acta crystallographica. Section F, Structural biology and crystallization communications · 2013Article
- Investigation of energy gene expressions and community structures of free and attached acidophilic bacteria in chalcopyrite bioleaching.Journal of industrial microbiology & biotechnology · 2012Article
- Archaeal tetrathionate hydrolase goes viral: secretion of a sulfur metabolism enzyme in the form of virus-like particles.Applied and environmental microbiology · 2012Article
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4 authors at 1 institution in 1 country.
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Abstract
Production of sulfur globules during sulfide or thiosulfate oxidation is a characteristic feature of some sulfur bacteria. Although their generation has been reported in Acidithiobacillus ferrooxidans, its mechanism of formation and deposition, as well as the physiological significance of these globules during sulfur compounds oxidation, are currently unknown. Under oxygen-sufficient conditions (OSC), A. ferrooxidans oxidizes thiosulfate to tetrathionate, which accumulates in the culture medium. Tetrathionate is then oxidized by a tetrathionate hydrolase (TTH) generating thiosulfate, elemental sulfur, and sulfate as final products. We report here a massive production of extracellular conspicuous sulfur globules in thiosulfate-grown A. ferrooxidans cultures shifted to oxygen-limiting conditions (OLC). Concomitantly with sulfur globule deposition, the extracellular concentration of tetrathionate greatly diminished and sulfite accumulated in the culture supernatant. A. ferrooxidans cellular TTH activity was negligible in OLC-incubated cells, indicating that this enzymatic activity was not responsible for tetrathionate disappearance. On the other hand, supernatants from both OSC- and OLC-incubated cells showed extracellular TTH activity, which most likely accounted for tetrathionate consumption in the culture medium. The extracellular TTH activity described here: (i) gives experimental support to the TTH-driven model for hydrophilic sulfur globule generation, (ii) explains the extracellular location of A. ferrooxidans sulfur deposits, and (iii) strongly suggests that the generation of sulfur globules in A. ferrooxidans corresponds to an early step during its adaptation to an anaerobic lifestyle.
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