ArticleAmerican journal of physiology. Cell physiology2011
Physiological and histological changes in skeletal muscle following in vivo gene transfer by electroporation.
Article in American journal of physiology. Cell physiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 30 citations in OpenAlex.
- CaMKIIγ/δ contributes to mitochondrial metabolic adaptation in skeletal muscle during endurance training.Molecular metabolism · 2026Article
- Exosomes as an Emerging Plasmid Delivery Vehicle for Gene Therapy.Pharmaceutics · 2023Review
- CORP: Gene delivery into murine skeletal muscle using in vivo electroporation.Journal of applied physiology (Bethesda, Md. : 1985) · 2022Review
- Targeting Muscle-Resident Single Cells ThroughFrontiers in physiology · 2022Article
- A novel murine muscle loading model to investigate Achilles musculotendinous adaptation.Journal of applied physiology (Bethesda, Md. : 1985) · 2021Article
- Definition of a Novel Plasmid-Based Gene Transfection Protocol of Mammalian Skeletal Muscles by Means of In Vivo Electroporation.International journal of molecular sciences · 2020Article
- Wnt7a Counteracts Cancer Cachexia.Molecular therapy oncolytics · 2020Article
- Minimally Invasive Muscle Embedding Generates Donor-Cell-Derived Muscle Fibers that Express Desmin and Dystrophin.Military medicine · 2020Article
- Keratin 18 is an integral part of the intermediate filament network in murine skeletal muscle.American journal of physiology. Cell physiology · 2020Article
- A Critical Review of Electroporation as A Plasmid Delivery System in Mouse Skeletal Muscle.International journal of molecular sciences · 2019Review
- Damaged muscle fibers might masquerade as hybrid fibers - a cautionary note on immunophenotyping mouse muscle with mouse monoclonal antibodies.European journal of histochemistry : EJH · 2018Article
- Diltiazem improves contractile properties of skeletal muscle in dysferlin-deficient BLAJ mice, but does not reduce contraction-induced muscle damage.Physiological reports · 2018Article
- Superparamagnetic Iron Oxide Nanoparticles in Musculoskeletal Biology.Tissue engineering. Part B, Reviews · 2017Article
- Plasma Membrane Repair in Health and Disease.Current topics in membranes · 2016Review
- DNA Electroporation, Isolation and Imaging of Myofibers.Journal of visualized experiments : JoVE · 2015Article
- Myofiber damage precedes macrophage infiltration after in vivo injury in dysferlin-deficient A/J mouse skeletal muscle.The American journal of pathology · 2015Article
- Optimizing in vivo gene transfer into mouse corpus cavernosum by use of surface electroporation.Korean journal of urology · 2015Article
- Intradermal delivery of Shigella IpaB and IpaD type III secretion proteins: kinetics of cell recruitment and antigen uptake, mucosal and systemic immunity, and protection across serotypes.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- Insulin- and contraction-induced glucose transporter 4 traffic in muscle: insights from a novel imaging approach.Exercise and sport sciences reviews · 2013Review
- Distinct effects of contraction-induced injury in vivo on four different murine models of dysferlinopathy.Journal of biomedicine & biotechnology · 2012Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Electroporation (EP) is used to transfect skeletal muscle fibers in vivo, but its effects on the structure and function of skeletal muscle tissue have not yet been documented in detail. We studied the changes in contractile function and histology after EP and the influence of the individual steps involved to determine the mechanism of recovery, the extent of myofiber damage, and the efficiency of expression of a green fluorescent protein (GFP) transgene in the tibialis anterior (TA) muscle of adult male C57Bl/6J mice. Immediately after EP, contractile torque decreased by ∼80% from pre-EP levels. Within 3 h, torque recovered to ∼50% but stayed low until day 3. Functional recovery progressed slowly and was complete at day 28. In muscles that were depleted of satellite cells by X-irradiation, torque remained low after day 3, suggesting that myogenesis is necessary for complete recovery. In unirradiated muscle, myogenic activity after EP was confirmed by an increase in fibers with central nuclei or developmental myosin. Damage after EP was confirmed by the presence of necrotic myofibers infiltrated by CD68+ macrophages, which persisted in electroporated muscle for 42 days. Expression of GFP was detected at day 3 after EP and peaked on day 7, with ∼25% of fibers transfected. The number of fibers expressing green fluorescent protein (GFP), the distribution of GFP+ fibers, and the intensity of fluorescence in GFP+ fibers were highly variable. After intramuscular injection alone, or application of the electroporating current without injection, torque decreased by ∼20% and ∼70%, respectively, but secondary damage at D3 and later was minimal. We conclude that EP of murine TA muscles produces variable and modest levels of transgene expression, causes myofiber damage due to the interaction of intramuscular injection with the permeabilizing current, and that full recovery requires myogenesis.
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