ArticleThe journal of histochemistry and cytochemistry : official journal of the Histochemistry Society2011
Localization of a wide-ranging panel of antigens in the rat retina by immunohistochemistry: comparison of Davidson's solution and formalin as fixatives.
Article in The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 49 citations in OpenAlex.
- Improvement and development of immersion fixatives for enhanced tissue preservation in the whole eyeball specimens.The Journal of veterinary medical science · 2025Article
- Evaluation of Glyoxal fixation for immunohistochemistry of the retina.Scientific reports · 2025Article
- Aquaporin-4 Peptide Injection in Mice Induces Retinal and Optic Nerve Alterations That Simulate Those of Neuromyelitis Optica Spectrum Disorder.Translational vision science & technology · 2025Article
- Multiparametric grading of glaucoma severity by histopathology can enable post-mortem substratification of disease state.Acta neuropathologica communications · 2025Article
- Investigation into the use of a chitosan scaffold for tarsal plate substitution in eyelid reconstruction.Regenerative biomaterials · 2025Article
- A chimeric anti-vascularization immunomodulator prevents high-risk corneal transplantation rejection via ex vivo gene therapy.Molecular therapy : the journal of the American Society of Gene Therapy · 2024Article
- Histochemical Comparison of Human and Rat Lacrimal Glands: Implications for Bio-Engineering Studies.Translational vision science & technology · 2022Article
- Differential Effects of Experimental Retinal Detachment on S- and M/L-Cones in Rats.Molecular neurobiology · 2022Article
- Investigations into photoreceptor energy metabolism during experimental retinal detachment.Frontiers in cellular neuroscience · 2022Article
- Safety Profile of Slit-Lamp-Delivered Retinal Laser Photobiomodulation.Translational vision science & technology · 2020Article
- Spatio-temporal characterization of S- and M/L-cone degeneration in the Rd1 mouse model of retinitis pigmentosa.BMC neuroscience · 2019Article
- Improved immunohistochemical detection of phosphorylated mitogen-activated protein kinases in the injured rat optic nerve head.Histochemistry and cell biology · 2019Article
- Investigations Into Bioenergetic Neuroprotection of Cone Photoreceptors: Relevance to Retinitis Pigmentosa.Frontiers in neuroscience · 2019Article
- Optimization of fixative solution for retinal morphology: a comparison with Davidson's fixative and other fixation solutions.Japanese journal of ophthalmology · 2018Article
- Investigations into Hypoxia and Oxidative Stress at the Optic Nerve Head in a Rat Model of Glaucoma.Frontiers in neuroscience · 2017Article
- Investigations into Retinal Pathology in the Early Stages of a Mouse Model of Alzheimer's Disease.Journal of Alzheimer's disease : JAD · 2017Article
- Expression and distribution of peroxiredoxins in the retina and optic nerve.Brain structure & function · 2016Article
- Comparison of histopathological findings between idiopathic and secondary epiretinal membranes.International ophthalmology · 2016Article
- Fixation strategies for retinal immunohistochemistry.Progress in retinal and eye research · 2015Review
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The preferred fixative for whole eyes is Davidson's solution, which provides optimal tissue preservation while avoiding retinal detachment. Hitherto, the compatibility of Davidson's solution with immunohistochemistry has been largely untested. The goal of the present study was to compare the immunolabeling patterns of a wide-ranging panel of commercially available, previously validated antibodies in formalin- and Davidson's-fixed retinas. Immunohistochemistry was performed in normal pigmented rat eyes and, to facilitate localization of inducible proteins, eyes injected with the bacterial toxin lipopolysaccharide or subjected to laser-induced photoreceptor damage. Specificity of labeling was judged by the morphology and distribution of immunopositive cells, by the absence of signal in appropriate controls, and by comparison with expected staining patterns. Retinas fixed in formalin displayed only adequate morphological integrity but were highly compatible with all 39 antibodies evaluated. Retinas fixed in Davidson's solution displayed morphological integrity superior to those fixed in formalin. Generally, the cellular and subcellular patterns and intensities of immunoreactivities obtained with each fixative were identical; however, Davidson's fixative was less compatible with certain antibodies, such as the neurotransmitter γ-aminobutyric acid, the microglial marker iba1, the macroglial stress protein nestin, and the small heat shock proteins Hsp27 and αB-crystallin, shortfalls that somewhat temper enthusiasm concerning its use.
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