ArticleJournal of clinical microbiology2011
Rapid and accurate identification of human-associated staphylococci by use of multiplex PCR.
Article in Journal of clinical microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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Who cites it
50 citing papers in PubMed.
- Plasmid diversity, antibiotic resistance and virulence genes associated with Staphylococcus aureus isolates from private hospitals in Gauteng, South Africa.World journal of microbiology & biotechnology · 2026Article
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- The prevalence of multidrug resistance in Staphylococcus hominis isolated from clinical materials.Scientific reports · 2025Article
- Prevalence, species identification, and antibiotic resistance of Staphylococci in dogs visiting veterinary clinics in Vietnam.PloS one · 2025Article
- The Opportunistic PathogenAntibiotics (Basel, Switzerland) · 2024Review
- Virulence, Susceptibility Profile, and Clinical Characteristics of Pathogenic Coagulase-Negative Staphylococci.Cureus · 2024Article
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- Association of coagulase-negative staphylococci with orthopedic infections detected by in-house multiplex real-time PCR.Frontiers in microbiology · 2024Article
- Spectrum of Virulence Factors in Clinical Isolates ofJournal of laboratory physicians · 2023Article
- Decreased susceptibility to vancomycin and other mechanisms of resistance to antibiotics in Staphylococcus epidermidis as a therapeutic problem in hospital treatment.Scientific reports · 2023Article
- The frequency of adherence, biofilm-associated, Arginine Catabolic Mobile element genes, and biofilm formation in clinical and healthcare worker coagulase-negative staphylococci isolates.BMC microbiology · 2023Article
- Subacute osteomyelitis due toWorld journal of clinical cases · 2023Article
- Analysis of the Presence of the Virulence and Regulation Genes fromInternational journal of environmental research and public health · 2023Article
- Risk factors for meticillin-resistant Staphylococcus aureus (MRSA) carriage in MRSA-exposed household pets.Veterinary dermatology · 2023Article
- Current Limitations of Staph Infection Diagnostics, and the Role for VOCs in Achieving Culture-Independent Detection.Pathogens (Basel, Switzerland) · 2023Review
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- Identification of major sequence types among aminoglycoside resistantIranian journal of microbiology · 2022Article
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- Characterization ofPeerJ · 2022Article
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although staphylococci are identified by phenotypic analysis in many clinical laboratories, these results are often incorrect because of phenotypic variation. Genetic analysis is necessary for definitive species identification. In the present study, we developed a simple multiplex-PCR (M-PCR) for species identification of human-associated staphylococci, which were as follows: Staphylococcus aureus, S. capitis, S. caprae, S. epidermidis, S. haemolyticus, S. hominis, S. lugdunensis, S. saprophyticus, and S. warneri. This method was designed on the basis of nucleotide sequences of the thermonuclease (nuc) genes that were universally conserved in staphylococci except the S. sciuri group and showed moderate sequence diversity. In order to validate this assay, 361 staphylococcal strains were studied, which had been identified at the species levels by sequence analysis of the hsp60 genes. In consequence, M-PCR demonstrated a sensitivity of 100% and a specificity of 100%. By virtue of simplicity and accuracy, this method will be useful in clinical research.
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