ArticleDevelopment (Cambridge, England)2011
Cell cycle arrest in node cells governs ciliogenesis at the node to break left-right symmetry.
Article in Development (Cambridge, England), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 21 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
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- Medullary Tegmental Cap Dysplasia: Fetal and Postnatal Presentations of a Unique Brainstem Malformation.AJNR. American journal of neuroradiology · 2023Review
- Agonists of prostaglandin EProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Elevated WNT signaling and compromised Hedgehog signaling due toFrontiers in dental medicine · 2022Article
- Generation of a new mouse line with conditionally activated signaling through the BMP receptor, ACVR1: A tool to characterize pleiotropic roles of BMP functions.Genesis (New York, N.Y. : 2000) · 2021Article
- Primary Cilia Formation Does Not Rely on WNT/β-Catenin Signaling.Frontiers in cell and developmental biology · 2021Article
- Chemokine signaling links cell-cycle progression and cilia formation for left-right symmetry breaking.PLoS biology · 2019Article
- A Cell/Cilia Cycle Biosensor for Single-Cell Kinetics Reveals Persistence of Cilia after G1/S Transition Is a General Property in Cells and Mice.Developmental cell · 2018Article
- TGF-β Family Signaling in Early Vertebrate Development.Cold Spring Harbor perspectives in biology · 2018Review
- BmpR1A is a major type 1 BMP receptor for BMP-Smad signaling during skull development.Developmental biology · 2017Article
- Functional Diversity of Ciliary Proteins in Bone Development and Disease.Current osteoporosis reports · 2017Review
- Article
- TGF-β and BMP signaling in osteoblast, skeletal development, and bone formation, homeostasis and disease.Bone research · 2016Review
- Generation of Evc2/Limbin global and conditional KO mice and its roles during mineralized tissue formation.Genesis (New York, N.Y. : 2000) · 2015Article
- The type I BMP receptor ACVR1/ALK2 is required for chondrogenesis during development.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2015Article
- BMP signaling is required for cell cleavage in preimplantation-mouse embryos.Developmental biology · 2015Article
- Nodal signaling promotes a tumorigenic phenotype in human breast cancer.Seminars in cancer biology · 2014Review
- Neural crest cell signaling pathways critical to cranial bone development and pathology.Experimental cell research · 2014Review
- Establishment of left-right asymmetry in vertebrate development: the node in mouse embryos.Cellular and molecular life sciences : CMLS · 2013Review
- Left-right asymmetry: lessons from Cancún.Development (Cambridge, England) · 2013Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Cilia at the node generate a leftward fluid flow that breaks left-right symmetry. However, the molecular mechanisms that regulate ciliogenesis at the node are largely unknown. Here, we show that the epiblast-specific deletion of the gene encoding the BMP type 1 receptor (Acvr1) compromised development of nodal cilia, which results in defects in leftward fluid flow and, thus, abnormalities in left-right patterning. Acvr1 deficiency in mouse embryonic fibroblasts (MEFs) resulted in severe defects in their quiescence-induced primary cilia. Although the induction of quiescence in wild-type MEFs leads to an increase in the level of the cyclin-dependent kinase inhibitor p27(Kip1) and to rapid p27(Kip1) phosphorylation on Ser(10), MEFs deficient in Acvr1 show a reduction in both p27(Kip1) protein levels and in p27(Kip1) Ser(10) phosphorylation. The observed defects in cilium development were rescued by the introduction of p27(Kip1) into Acvr1-deficient MEFs, implying that BMP signaling positively controls p27(Kip1) stability in the G0 phase via p27(Kip1) Ser(10) phosphorylation, which is a prerequisite for induction of primary cilia. Importantly, in control embryos, p27(Kip1) protein is clearly present and strongly phosphorylated on Ser(10) in cells on the quiescent ventral surface of the node. By contrast, the corresponding cells in the node of Acvr1 mutant embryos were proliferative and showed a dramatic attenuation in both p27(Kip1) protein levels and phosphorylation on Ser(10). Our data suggest that cell quiescence controlled by BMP signaling via ACVR1 is required for transient formation of nodal cilia, and provide insight into the fundamental question of how the node represents the mechanistic `node' that regulates the development of left-right symmetry in vertebrates.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.