ArticleMolecular human reproduction2011
Cleavage of rRNA ensures translational cessation in sperm at fertilization.
Article in Molecular human reproduction, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
49 citing papers in PubMed, 120 citations in OpenAlex.
- Sperm 28S ribosomal RNA fragments as a potential biomarker for embryo quality in in vitro fertilization.Journal of assisted reproduction and genetics · 2026Article
- Epididymal extracellular vesicles harbor and convey mRNA to sperm for transfer to zygotes.Nucleic acids research · 2026Article
- TRiC Is a Structural Component of Mammalian Sperm Axonemes.Cytoskeleton (Hoboken, N.J.) · 2025Review
- Copy number determination of sperm-borne small RNAs implied in the intergenerational inheritance of metabolic syndromes.RNA (New York, N.Y.) · 2025Article
- RNA Sequencing of Sperm from Healthy Cattle and Horses Reveals the Presence of a Large Bacterial Population.Current issues in molecular biology · 2024Article
- Small RNAs: an ideal choice as sperm quality biomarkers.Frontiers in reproductive health · 2024Article
- Missing Causality and Heritability of Autoimmune Hepatitis.Digestive diseases and sciences · 2023Article
- Men who inject opioids exhibit altered tRNA-Gly-GCC isoforms in semen.Molecular human reproduction · 2023Article
- Age-associated epigenetic changes in mammalian sperm: implications for offspring health and development.Human reproduction update · 2023Review
- Assessing spermatozoal small ribonucleic acids and their relationship to blastocyst development in idiopathic infertile males.Scientific reports · 2022Article
- Soma-to-germline RNA communication.Nature reviews. Genetics · 2022Review
- The ribosome inhibitor chloramphenicol induces motility deficits in human spermatozoa: A proteomic approach identifies potentially involved proteins.Frontiers in cell and developmental biology · 2022Article
- Single-molecule long-read sequencing reveals a conserved intact long RNA profile in sperm.Nature communications · 2021Article
- Roles for the RNA polymerase III regulator MAFR-1 in regulating sperm quality in Caenorhabditis elegans.Scientific reports · 2020Article
- Repeated sampling facilitates within- and between-subject modeling of the human sperm transcriptome to identify dynamic and stress-responsive sncRNAs.Scientific reports · 2020Article
- A comprehensive overview of bull sperm-borne small non-coding RNAs and their diversity across breeds.Epigenetics & chromatin · 2020Article
- Article
- Single-cell sperm transcriptomes and variants from fathers of children with and without autism spectrum disorder.NPJ genomic medicine · 2020Article
- Article
- Mature sperm small-RNA profile in the sparrow: implications for transgenerational effects of age on fitness.Environmental epigenetics · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Intact ribosomal RNAs (rRNAs) comprise the majority of somatic transcripts, yet appear conspicuously absent in spermatozoa, perhaps reflecting cytoplasmic expulsion during spermatogenesis. To discern their fate, total RNA retained in mature spermatozoa from three fertile donors was characterized by Next Generation Sequencing. In all samples, >75% of total sequence reads aligned to rRNAs. The distribution of reads along the length of these transcripts exhibited a high degree of non-uniformity that was reiterated between donors. The coverage of sequencing reads was inversely correlated with guanine-cytosine (GC)-richness such that sequences greater than ∼70% GC were virtually absent in all sperm RNA samples. To confirm the loss of sequence, the relative abundance of specific regions of the 28S transcripts in sperm was established by 7-Deaza-2'-deoxy-guanosine-5'-triphosphate RT-PCR. The inability to amplify specific regions of the 28S sequence from sperm despite the abundant representation of this transcript in the sequencing libraries demonstrates that approximately three-quarters of RNA retained in the mature male gamete are products of rRNA fragmentation. Hence, cleavage (not expulsion of the RNA component of the translational machinery) is responsible for preventing spurious translation following spermiogenesis. These results highlight the potential importance of those transcripts, including many mRNAs, which evade fragmentation and remain intact when sperm are delivered at fertilization. Sequencing data are deposited in GEO as: GSE29160.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.