ArticleMolecular plant pathology2012
Genetic characterization of the HrpL regulon of the fire blight pathogen Erwinia amylovora reveals novel virulence factors.
Article in Molecular plant pathology, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 68 citations in OpenAlex.
- Pathogenicity and virulence ofVirulence · 2026Review
- Tizoxanide associated with anti-virulent activity controls fire blight disease caused by Erwinia amylovora in Malus asiatica.Communications biology · 2025Article
- Osmotic and pH Stress-Responsive Two-Component System, OmpR/EnvZ, Modulates Type III Secretion, Biofilm Formation, Swimming Motility and Virulence in Acidovorax citrulli xjL12.Molecular plant pathology · 2025Article
- Manuka Honey Inhibits Biofilm Formation and Reduces the Expression of the Associated Genes inScientifica · 2024Article
- Comprehensive genome sequence analysis ofFrontiers in microbiology · 2024Article
- Identification of novel virulence factors in Erwinia amylovora through temporal transcriptomic analysis of infected apple flowers under field conditions.Molecular plant pathology · 2022Article
- Five Plant Natural Products Are Potential Type III Secretion System Inhibitors to Effectively Control Soft-Rot Disease Caused byFrontiers in microbiology · 2022Article
- HrpL Regulon of Bacterial Pathogen of Woody HostMicroorganisms · 2021Article
- Orchestration of virulence factor expression and modulation of biofilm dispersal in Erwinia amylovora through activation of the Hfq-dependent small RNA RprA.Molecular plant pathology · 2021Article
- Multi-Omics Analysis Reveals the Dynamic Changes of RNA NFrontiers in microbiology · 2021Article
- Phosphodiesterase Genes Regulate Amylovoran Production, Biofilm Formation, and Virulence in Erwinia amylovora.Applied and environmental microbiology · 2019Article
- Lon protease modulates virulence traits in Erwinia amylovora by direct monitoring of major regulators and indirectly through the Rcs and Gac-Csr regulatory systems.Molecular plant pathology · 2018Article
- Integration of multiple stimuli-sensing systems to regulate HrpS and type III secretion system in Erwinia amylovora.Molecular genetics and genomics : MGG · 2018Article
- Transcriptional response of Erwinia amylovora to copper shock: in vivo role of the copA gene.Molecular plant pathology · 2018Article
- Comparative transcriptome analysis of a lowly virulent strain of Erwinia amylovora in shoots of two apple cultivars - susceptible and resistant to fire blight.BMC genomics · 2017Article
- The RNA-binding protein CsrA plays a central role in positively regulating virulence factors in Erwinia amylovora.Scientific reports · 2016Article
- Identification of the HrpS binding site in the hrpL promoter and effect of the RpoN binding site of HrpS on the regulation of the type III secretion system in Erwinia amylovora.Molecular plant pathology · 2016Article
- Article
- The AvrE superfamily: ancestral type III effectors involved in suppression of pathogen-associated molecular pattern-triggered immunity.Molecular plant pathology · 2015Review
- The bacterial alarmone (p)ppGpp activates the type III secretion system in Erwinia amylovora.Journal of bacteriology · 2015Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
Abstract
The bacterial pathogen Erwinia amylovora is the causal agent of fire blight, an economically significant disease of apple and pear. Disease initiation by E. amylovora requires the translocation of effector proteins into host cells via the hypersensitive response and pathogenicity (hrp) type III secretion system (T3SS). The alternative sigma factor HrpL positively regulates the transcription of structural and translocated components of the T3SS via hrp promoter elements. To characterize genome-wide HrpL-dependent gene expression in E. amylovora Ea1189, wild-type and Ea1189ΔhrpL strains were cultured in hrp-inducing minimal medium, and total RNA was compared using a custom microarray designed to represent the annotated genes of E. amylovora ATCC 49946. The results revealed 24 genes differentially regulated in Ea1189ΔhrpL relative to Ea1189 with fold-change expression ratios greater than 1.5; of these, 19 genes exhibited decreased transcript abundance and five genes showed increased transcript abundance relative to Ea1189. To expand our understanding of the HrpL regulon and to elucidate direct versus indirect HrpL-mediated effects on gene expression, the genome of E. amylovora ATCC 49946 was examined in silico using a hidden Markov model assembled from known Erwinia spp. hrp promoters. This technique identified 15 putative type III novel hrp promoters, seven of which were validated with quantitative polymerase chain reaction based on expression analyses. It was found that HrpL-regulated genes encode all known components of the hrp T3SS, as well as five putative type III effectors. Eight genes displayed apparent indirect HrpL regulation, suggesting that the HrpL regulon is connected to downstream signalling networks. The construction of deletion mutants of three novel HrpL-regulated genes resulted in the identification of additional virulence factors as well as mutants displaying abnormal motility and biofilm phenotypes.
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