ArticleLipids2012
Steryl glucoside and acyl steryl glucoside analysis of Arabidopsis seeds by electrospray ionization tandem mass spectrometry.
Article in Lipids, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 46 citations in OpenAlex.
- From Green to Ripe: Untargeted UHPLC-Orbitrap-MS Lipidomic Profiling of Cacao Seed and Pod Husk during Maturation.Journal of agricultural and food chemistry · 2026Article
- Sterols in plant biologyCell surface (Amsterdam, Netherlands) · 2025Review
- Lipid modulation contributes to heat stress adaptation in peanut.Frontiers in plant science · 2023Article
- Sterylglucosides in Fungi.Journal of fungi (Basel, Switzerland) · 2022Review
- Article
- Chemical composition of the lipophilic compounds from the rind and pith of papyrus (Frontiers in plant science · 2022Article
- Leaf Lipid Alterations in Response to Heat Stress ofPlants (Basel, Switzerland) · 2020Article
- Development of Methods to Quantify Free and Conjugated Steroids in Fatty Matrices by HPLC-MS/MS.ACS omega · 2020Article
- UPLC-MS/MS Based Identification of Dietary Steryl Glucosides by Investigation of Corresponding Free Sterols.Frontiers in chemistry · 2018Article
- Positioning of the SCRAMBLED receptor requires UDP-Glc:sterol glucosyltransferase 80B1 in Arabidopsis roots.Scientific reports · 2017Article
- Lipid profiles of detergent resistant fractions of the plasma membrane in oat and rye in association with cold acclimation and freezing tolerance.Cryobiology · 2016Article
- Differential effect of plant lipids on membrane organization: specificities of phytosphingolipids and phytosterols.The Journal of biological chemistry · 2015Article
- Functional diversification of two UGT80 enzymes required for steryl glucoside synthesis in Arabidopsis.Journal of experimental botany · 2015Article
- Plasma membrane lipid-protein interactions affect signaling processes in sterol-biosynthesis mutants in Arabidopsis thaliana.Frontiers in plant science · 2014Article
- Plant phloem sterol content: forms, putative functions, and implications for phloem-feeding insects.Frontiers in plant science · 2013Article
- Deciphering the molecular functions of sterols in cellulose biosynthesis.Frontiers in plant science · 2012Article
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Establishment of sensitive methods for the detection of cellular sterols and their derivatives is a critical step in developing comprehensive lipidomics technology. We demonstrate that electrospray ionization tandem (triple quadrupole) mass spectrometry (ESI-MS/MS) is an efficient method for monitoring steryl glucosides (SG) and acyl steryl glucosides (ASG). Comparison of analysis of SG and ASG by ESI-MS/MS with analysis by gas chromatography with flame ionization detection (GC-FID) shows that the two methods yield similar molar compositions. These data demonstrate that ESI-MS/MS response per molar amount of sterol conjugate is similar among various molecular species of SG and ASG. Application of ESI-MS/MS to seed samples from wild-type Arabidopsis and a mutant deficient in two UDP-glucose:sterol glucosyltransferases, UGT80A2 and UGT80B1, revealed new details on the composition of sitosteryl, campesteryl and stigmasteryl glucosides and ASG. SG were decreased by 86% in the ugt80A2,B1 double mutant, compared to the wild-type, while ASG were reduced 96%. The results indicate that these glucosyltransferases account for much of the accumulation of the sterol conjugates in wild-type Arabidopsis seeds.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.