ArticleTAG. Theoretical and applied genetics. Theoretische und angewandte Genetik2011
PCR-based isolation and identification of full-length low-molecular-weight glutenin subunit genes in bread wheat (Triticum aestivum L.).
Article in TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 27 citations in OpenAlex.
- Characterization and Classification of LMW-GS Genes at theInternational journal of molecular sciences · 2025Article
- Recent duplications and rare structural variations revealed by comparative sequence analysis of low molecular weight glutenin subunits (LMW-GS) genes re-identified using LMWgsFinder in 26 genomes of the grass family.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- A telomere-to-telomere genome assembly coupled with multi-omic data provides insights into the evolution of hexaploid bread wheat.Nature genetics · 2025Article
- Comparison and Classification of LMW-GS Genes atGenes · 2025Article
- The Landscapes of Gluten Regulatory Network in Elite Wheat Cultivars Contrasting in Gluten Strength.International journal of molecular sciences · 2023Article
- The endosperm-specific transcription factor TaNAC019 regulates glutenin and starch accumulation and its elite allele improves wheat grain quality.The Plant cell · 2021Article
- The genetic diversity of group-1 homoeologs and characterization of novel LMW-GS genes from Chinese Xinjiang winter wheat landraces (Triticum aestivum L.).Journal of applied genetics · 2020Article
- Low molecular weight glutenin subunit gene composition at Glu-D3 loci of Aegilops tauschii and common wheat and a further view of wheat evolution.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2018Article
- Evidence of intralocus recombination at the Glu-3 loci in bread wheat (Triticum aestivum L.).TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2017Article
- Allelic variation of LMW-GS composition in Chinese wheat landraces of the Yangtze-River region detected by MALDI-TOF-MS.Breeding science · 2016Article
- Molecular characterization of novel LMW-i glutenin subunit genes from Triticum urartu Thum. ex Gandil.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2015Article
- Composition, variation, expression and evolution of low-molecular-weight glutenin subunit genes in Triticum urartu.BMC plant biology · 2015Article
- Novel LMW glutenin subunit genes from wild emmer wheat (Triticum turgidum ssp. dicoccoides) in relation to Glu-3 evolution.Development genes and evolution · 2015Article
- Cloning and characterization of low-molecular-weight glutenin subunit alleles from Chinese wheat landraces (Triticum aestivum L.).TheScientificWorldJournal · 2014Article
- Novel insights into the composition, variation, organization, and expression of the low-molecular-weight glutenin subunit gene family in common wheat.Journal of experimental botany · 2013Article
- Composition and functional analysis of low-molecular-weight glutenin alleles with Aroona near-isogenic lines of bread wheat.BMC plant biology · 2012Article
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8 authors at 4 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Low-molecular-weight glutenin subunits (LMW-GSs) are encoded by a multi-gene family and are essential for determining the quality of wheat flour products, such as bread and noodles. However, the exact role or contribution of individual LMW-GS genes to wheat quality remains unclear. This is, at least in part, due to the difficulty in characterizing complete sequences of all LMW-GS gene family members in bread wheat. To identify full-length LMW-GS genes, a polymerase chain reaction (PCR)-based method was established, consisting of newly designed conserved primers and the previously developed LMW-GS gene molecular marker system. Using the PCR-based method, 17 LMW-GS genes were identified and characterized in Xiaoyan 54, of which 12 contained full-length sequences. Sequence alignments showed that 13 LMW-GS genes were identical to those found in Xiaoyan 54 using the genomic DNA library screening, and the other four full-length LMW-GS genes were first isolated from Xiaoyan 54. In Chinese Spring, 16 unique LMW-GS genes were isolated, and 13 of them contained full-length coding sequences. Additionally, 16 and 17 LMW-GS genes in Dongnong 101 and Lvhan 328 (chosen from the micro-core collections of Chinese germplasm), respectively, were also identified. Sequence alignments revealed that at least 15 LMW-GS genes were common in the four wheat varieties, and allelic variants of each gene shared high sequence identities (>95%) but exhibited length polymorphism in repetitive regions. This study provides a PCR-based method for efficiently identifying LMW-GS genes in bread wheat, which will improve the characterization of complex members of the LMW-GS gene family and facilitate the understanding of their contributions to wheat quality.
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