ArticlePloS one2011
Proteomic analysis reveals that iron availability alters the metabolic status of the pathogenic fungus Paracoccidioides brasiliensis.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers.
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Who cites it
38 citing papers in PubMed, 70 citations in OpenAlex.
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- Iron Deprivation Modulates the Exoproteome inFrontiers in cellular and infection microbiology · 2022Article
- Interacting with Hemoglobin:Journal of fungi (Basel, Switzerland) · 2021Article
- Prediction of Conserved Peptides ofJournal of fungi (Basel, Switzerland) · 2020Article
- The "Little Iron Waltz": The Ternary Response ofJournal of fungi (Basel, Switzerland) · 2020Review
- The genome of opportunistic fungal pathogen Fusarium oxysporum carries a unique set of lineage-specific chromosomes.Communications biology · 2020Article
- Living Within the Macrophage: Dimorphic Fungal Pathogen Intracellular Metabolism.Frontiers in cellular and infection microbiology · 2020Review
- The Trojan Horse Model inFrontiers in cellular and infection microbiology · 2020Review
- Molecular characterization of siderophore biosynthesis inIMA fungus · 2020Article
- Propionate metabolism in a human pathogenic fungus: proteomic and biochemical analyses.IMA fungus · 2020Article
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- Serological proteomic biomarkers to identify Paracoccidioides species and risk of relapse.PloS one · 2018Article
- Identification of membrane proteome ofFuture science OA · 2017Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Paracoccidioides brasiliensis is a thermodimorphic fungus and the causative agent of paracoccidioidomycosis (PCM). The ability of P. brasiliensis to uptake nutrients is fundamental for growth, but a reduction in the availability of iron and other nutrients is a host defense mechanism many pathogenic fungi must overcome. Thus, fungal mechanisms that scavenge iron from host may contribute to P. brasiliensis virulence. In order to better understand how P. brasiliensis adapts to iron starvation in the host we compared the two-dimensional (2D) gel protein profile of yeast cells during iron starvation to that of iron rich condition. Protein spots were selected for comparative analysis based on the protein staining intensity as determined by image analysis. A total of 1752 protein spots were selected for comparison, and a total of 274 out of the 1752 protein spots were determined to have changed significantly in abundance due to iron depletion. Ninety six of the 274 proteins were grouped into the following functional categories; energy, metabolism, cell rescue, virulence, cell cycle, protein synthesis, protein fate, transcription, cellular communication, and cell fate. A correlation between protein and transcript levels was also discovered using quantitative RT-PCR analysis from RNA obtained from P. brasiliensis under iron restricting conditions and from yeast cells isolated from infected mouse spleens. In addition, western blot analysis and enzyme activity assays validated the differential regulation of proteins identified by 2-D gel analysis. We observed an increase in glycolytic pathway protein regulation while tricarboxylic acid cycle, glyoxylate and methylcitrate cycles, and electron transport chain proteins decreased in abundance under iron limiting conditions. These data suggest a remodeling of P. brasiliensis metabolism by prioritizing iron independent pathways.
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