ArticlePloS one2011
A sensitive high-throughput assay for evaluating host-pathogen interactions in Cryptococcus neoformans infection.
Article in PloS one, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
25 citing papers in PubMed.
- Article
- Distinct STRIPAK subunits drive conserved and subunit-specific signaling programs inbioRxiv : the preprint server for biology · 2026Article
- Use of a human immortalized microglia cell line to study recognition, phagocytosis, and intracellular survival ofmSphere · 2026Article
- Article
- Mapping the transcriptional regulatory network of a fungal pathogen by exploiting transcription factor perturbation.bioRxiv : the preprint server for biology · 2025Article
- Role of the Anaphase-Promoting Complex Activator Cdh1 in the Virulence ofJournal of fungi (Basel, Switzerland) · 2024Article
- Exposure of Cryptococcus neoformans to low nitrogen levels enhances virulence.International microbiology : the official journal of the Spanish Society for Microbiology · 2024Article
- Article
- A Quick reCAP: DiscoveringJournal of fungi (Basel, Switzerland) · 2024Article
- Assessing Phagocytosis of Cryptococcus neoformans Cells in Human Monocytes or the J774 Murine Macrophage Cell Line.Methods in molecular biology (Clifton, N.J.) · 2024Article
- The Environmental Effects on Virulence Factors and the Antifungal Susceptibility ofInternational journal of molecular sciences · 2021Review
- Dangerous Liaisons: Interactions ofPathogens (Basel, Switzerland) · 2020Review
- An Automated Assay to Measure Phagocytosis of Cryptococcus neoformans.Current protocols in microbiology · 2019Article
- Cdk8 and Ssn801 Regulate Oxidative Stress Resistance and Virulence in Cryptococcus neoformans.mBio · 2019Article
- Article
- RNA Interference Screening Reveals Host CaMK4 as a Regulator of Cryptococcal Uptake and Pathogenesis.Infection and immunity · 2017Article
- A fluorogenic C. neoformans reporter strain with a robust expression of m-cherry expressed from a safe haven site in the genome.Fungal genetics and biology : FG & B · 2017Article
- Interaction of THP-1 Monocytes with Conidia and Hyphae of DifferentFrontiers in immunology · 2017Article
- Cryptococcus neoformans UGT1 encodes a UDP-Galactose/UDP-GalNAc transporter.Glycobiology · 2017Article
- Article
Corrections and comments
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Authors and funding
4 authors.
Funding
Abstract
backgroundCryptococcus neoformans causes serious disease in immunocompromised individuals, leading to over 600,000 deaths per year worldwide. Part of this impact is due to the organism's ability to thwart what should be the mammalian hosts' first line of defense against cryptococcal infection: internalization by macrophages. Even when C. neoformans is engulfed by host phagocytes, it can survive and replicate within them rather than being destroyed; this ability is central in cryptococcal virulence. It is therefore critical to elucidate the interactions of this facultative intracellular pathogen with phagocytic cells of its mammalian host. METHODOLOGY/PRINCIPAL
findingsTo accurately assess initial interactions between human phagocytic cells and fungi, we have developed a method using high-throughput microscopy to efficiently distinguish adherent and engulfed cryptococci and quantitate each population. This method offers significant advantages over currently available means of assaying host-fungal cell interactions, and remains statistically robust when implemented in an automated fashion appropriate for screening. It was used to demonstrate the sensitivity of human phagocytes to subtle changes in the cryptococcal capsule, a major virulence factor of this pathogen. CONCLUSIONS/SIGNIFICANCE: Our high-throughput method for characterizing interactions between C. neoformans and mammalian phagocytic cells offers a powerful tool for elucidating the relationship between these cell types during pathogenesis. This approach will be useful for screens of this organism and has potentially broad applications for investigating host-pathogen interactions.
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Registered trials
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