ArticlePLoS genetics2011
Colon stem cell and crypt dynamics exposed by cell lineage reconstruction.
Article in PLoS genetics, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 37 papers.
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Who cites it
37 citing papers in PubMed.
- Computational approaches for multimodal lineage tracing.Nature reviews. Genetics · 2026Review
- Cell-to-cell variability and gain of methylation at polycomb CpG islands as a hallmark of aging.Nature communications · 2026Article
- Single-cell mitochondrial lineage tracing: Opportunities and challenges.Quantitative biology (Beijing, China) · 2026Review
- Review
- Lineage Tracing by Single-Cell Transcriptomics Decoding Dynamics of Lineage Commitment.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Best Practices in Designing, Sequencing, and Identifying Random DNA Barcodes.Journal of molecular evolution · 2023Review
- Temporal modelling using single-cell transcriptomics.Nature reviews. Genetics · 2022Review
- Replicative history marks transcriptional and functional disparity in the CD8Nature immunology · 2022Article
- Applications of Single-Cell DNA Sequencing.Annual review of genomics and human genetics · 2021Review
- Single-Cell Sequencing and Organoids: A Powerful Combination for Modelling Organ Development and Diseases.Reviews of physiology, biochemistry and pharmacology · 2021Review
- The Intestinal Epithelium - Fluid Fate and Rigid Structure From Crypt Bottom to Villus Tip.Frontiers in cell and developmental biology · 2021Review
- Identification of a subpopulation of long-term tumor-initiating cells in colon cancer.Bioscience reports · 2020Article
- Unravelling cellular relationships during development and regeneration using genetic lineage tracing.Nature reviews. Molecular cell biology · 2019Review
- Sample path properties of the average generation of a Bellman-Harris process.Journal of mathematical biology · 2019Article
- Lineage tracing using a Cas9-deaminase barcoding system targeting endogenous L1 elements.Nature communications · 2019Article
- Lineage Tracing: Computational Reconstruction Goes Beyond the Limit of Imaging.Molecules and cells · 2019Review
- Article
- Quantitative Analysis of Synthetic Cell Lineage Tracing Using Nuclease Barcoding.ACS synthetic biology · 2017Article
- Building a lineage from single cells: genetic techniques for cell lineage tracking.Nature reviews. Genetics · 2017Review
- The role of the Hes1 crosstalk hub in Notch-Wnt interactions of the intestinal crypt.PLoS computational biology · 2017Article
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stem cell dynamics in vivo are often being studied by lineage tracing methods. Our laboratory has previously developed a retrospective method for reconstructing cell lineage trees from somatic mutations accumulated in microsatellites. This method was applied here to explore different aspects of stem cell dynamics in the mouse colon without the use of stem cell markers. We first demonstrated the reliability of our method for the study of stem cells by confirming previously established facts, and then we addressed open questions. Our findings confirmed that colon crypts are monoclonal and that, throughout adulthood, the process of monoclonal conversion plays a major role in the maintenance of crypts. The absence of immortal strand mechanism in crypts stem cells was validated by the age-dependent accumulation of microsatellite mutations. In addition, we confirmed the positive correlation between physical and lineage proximity of crypts, by showing that the colon is separated into small domains that share a common ancestor. We gained new data demonstrating that colon epithelium is clustered separately from hematopoietic and other cell types, indicating that the colon is constituted of few progenitors and ruling out significant renewal of colonic epithelium from hematopoietic cells during adulthood. Overall, our study demonstrates the reliability of cell lineage reconstruction for the study of stem cell dynamics, and it further addresses open questions in colon stem cells. In addition, this method can be applied to study stem cell dynamics in other systems.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.