ArticleCirculation. Arrhythmia and electrophysiology2011
Stimulating cardiac muscle by light: cardiac optogenetics by cell delivery.
Article in Circulation. Arrhythmia and electrophysiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 111 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
111 citing papers in PubMed, 206 citations in OpenAlex.
- Crosstalk-free optical indicator-actuator combinations for bidirectional optogenetics.npj biomedical innovations · 2026Review
- Electrical coupling between transplanted cardiomyocytes and host myocardium to prevent arrhythmia.Disease models & mechanisms · 2026Review
- Phase resetting in human stem cell derived cardiomyocytes explains complex cardiac arrhythmias.PLoS computational biology · 2026Article
- XPRESSO: Rapid genetic engineering of human pluripotent stem cells for durable overexpression using a modular anti-silencing vector.Stem cell reports · 2025Article
- Complementary biomolecular coassemblies direct energy transport for cardiac photostimulators.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Optogenetic stimulation and simultaneous optical mapping of membrane potential and calcium transients in human engineered cardiac spheroids.Journal of molecular and cellular cardiology · 2025Article
- Monitoring and modulating cardiac bioelectricity: from Einthoven to end-user.Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology · 2024Review
- Nanotechnology-Fortified Manipulation of Cell CaSmall science · 2024Article
- Optical mapping and optogenetics in cardiac electrophysiology research and therapy: a state-of-the-art review.Europace : European pacing, arrhythmias, and cardiac electrophysiology : journal of the working groups on cardiac pacing, arrhythmias, and cardiac cellular electrophysiology of the European Society of Cardiology · 2024Review
- Cardiac optogenetics: shining light on signaling pathways.Pflugers Archiv : European journal of physiology · 2023Review
- OptoDyCE-plate as an affordable high throughput imager for all optical cardiac electrophysiology.Journal of molecular and cellular cardiology plus · 2023Article
- Modulating cardiac physiology in engineered heart tissue with the bidirectional optogenetic tool BiPOLES.Pflugers Archiv : European journal of physiology · 2023Article
- A micro-LED array based platform for spatio-temporal optogenetic control of various cardiac models.Scientific reports · 2023Article
- Opto-SICM framework combines optogenetics with scanning ion conductance microscopy for probing cell-to-cell contacts.Communications biology · 2023Article
- OptoDyCE-plate as an affordable high throughput imager for all optical cardiac electrophysiology.bioRxiv : the preprint server for biology · 2023Article
- Optogenetic-Induced Muscle Loading Leads to Mechanical Adaptation of the Achilles Tendon Enthesis in Mice.bioRxiv : the preprint server for biology · 2023Article
- Portable low-cost macroscopic mapping system for all-optical cardiac electrophysiology.Journal of biomedical optics · 2023Article
- A bibliometric profile of optogenetics: quantitative and qualitative analyses.Frontiers in neuroscience · 2023Article
- Optogenetic Control of Human Induced Pluripotent Stem Cell-Derived Cardiac Tissue Models.Journal of the American Heart Association · 2022Article
- The Integration of Optical Stimulation in a Mechanically Dynamic Cell Culture Substrate.Frontiers in bioengineering and biotechnology · 2022Article
51 more citing papers are in PubMed but not listed here.
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
backgroundAfter the recent cloning of light-sensitive ion channels and their expression in mammalian cells, a new field, optogenetics, emerged in neuroscience, allowing for precise perturbations of neural circuits by light. However, functionality of optogenetic tools has not been fully explored outside neuroscience, and a nonviral, nonembryogenesis-based strategy for optogenetics has not been shown before. METHODS AND
resultsWe demonstrate the utility of optogenetics to cardiac muscle by a tandem cell unit (TCU) strategy, in which nonexcitable cells carry exogenous light-sensitive ion channels, and, when electrically coupled to cardiomyocytes, produce optically excitable heart tissue. A stable channelrhodopsin2 (ChR2)-expressing cell line was developed, characterized, and used as a cell delivery system. The TCU strategy was validated in vitro in cell pairs with adult canine myocytes (for a wide range of coupling strengths) and in cardiac syncytium with neonatal rat cardiomyocytes. For the first time, we combined optical excitation and optical imaging to capture light-triggered muscle contractions and high-resolution propagation maps of light-triggered electric waves, found to be quantitatively indistinguishable from electrically triggered waves.
conclusionsOur results demonstrate feasibility to control excitation and contraction in cardiac muscle by light, using the TCU approach. Optical pacing in this case uses less energy, offers superior spatiotemporal control and remote access and can serve not only as an elegant tool in arrhythmia research but may form the basis for a new generation of light-driven cardiac pacemakers and muscle actuators. The TCU strategy is extendable to (nonviral) stem cell therapy and is directly relevant to in vivo applications.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.