ArticleThe Plant cell2011
Plastid uridine salvage activity is required for photoassimilate allocation and partitioning in Arabidopsis.
Article in The Plant cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 35 citations in OpenAlex.
- Uridine Kinase-like Protein (GhUKL4) Positively Regulates Resistance to Verticillium Wilt in Cotton.Genes · 2025Article
- Metabolomic Analysis Provides Insights into Bud Paradormancy inInternational journal of molecular sciences · 2025Article
- Chloroplastic ascorbate modifies plant metabolism and may act as a metabolite signal regardless of oxidative stress.Plant physiology · 2024Article
- Gene Functional Networks from Time Expression Profiles: A Constructive Approach Demonstrated in Chili Pepper (Plants (Basel, Switzerland) · 2023Article
- A plastid nucleoside kinase is involved in inosine salvage and control of purine nucleotide biosynthesis.The Plant cell · 2023Article
- Transcriptional reprogramming of nucleotide metabolism in response to altered pyrimidine availability in Arabidopsis seedlings.Frontiers in plant science · 2023Article
- The nucleotide metabolome of germinating Arabidopsis thaliana seeds reveals a central role for thymidine phosphorylation in chloroplast development.The Plant cell · 2022Article
- Light Intensity- and Spectrum-Dependent Redox Regulation of Plant Metabolism.Antioxidants (Basel, Switzerland) · 2022Review
- Metabolic Labeling of RNAs Uncovers Hidden Features and Dynamics of the Arabidopsis Transcriptome.The Plant cell · 2020Article
- Nucleotide Metabolism in Plants.Plant physiology · 2020Article
- Article
- Primary Metabolite Responses to Oxidative Stress in Early-Senescing and Paraquat ResistantFrontiers in plant science · 2020Article
- UMP Pyrophosphorylase: A Moonlighting Protein with Essential Functions in Chloroplast Development and Photosynthesis Establishment.Plant physiology · 2019Article
- Pyrimidine Salvage: Physiological Functions and Interaction with Chloroplast Biogenesis.Plant physiology · 2019Article
- UMP Kinase Regulates Chloroplast Development and Cold Response in Rice.International journal of molecular sciences · 2019Article
- Integrated RNA-seq and sRNA-seq analysis reveals miRNA effects on secondary metabolism in Solanum tuberosum L.Molecular genetics and genomics : MGG · 2017Article
- Of the Nine Cytidine Deaminase-Like Genes in Arabidopsis, Eight Are Pseudogenes and Only One Is Required to Maintain Pyrimidine Homeostasis in Vivo.Plant physiology · 2016Article
- Article
- Article
- Unknown components of the plastidial permeome.Frontiers in plant science · 2014Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nucleotides are synthesized from de novo and salvage pathways. To characterize the uridine salvage pathway, two genes, UKL1 and UKL2, that tentatively encode uridine kinase (UK) and uracil phosphoribosyltransferase (UPRT) bifunctional enzymes were studied in Arabidopsis thaliana. T-DNA insertions in UKL1 and UKL2 reduced transcript expression and increased plant tolerance to toxic analogs 5-fluorouridine and 5-fluorouracil. Enzyme activity assays using purified recombinant proteins indicated that UKL1 and UKL2 have UK but not UPRT activity. Subcellular localization using a C-terminal enhanced yellow fluorescent protein fusion indicated that UKL1 and UKL2 localize to plastids. The ukl2 mutant shows reduced transient leaf starch during the day. External application of orotate rescued this phenotype in ukl2, indicating pyrimidine pools are limiting for starch synthesis in ukl2. Intermediates for lignin synthesis were upregulated, and there was increased lignin and reduced cellulose content in the ukl2 mutant. Levels of ATP, ADP, ADP-glucose, UTP, UDP, and UDP-glucose were altered in a light-dependent manner. Seed composition of the ukl1 and ukl2 mutants included lower oil and higher protein compared with the wild type. Unlike single gene mutants, the ukl1 ukl2 double mutant has severe developmental defects and reduced biomass accumulation, indicating these enzymes catalyze redundant reactions. These findings point to crucial roles played by uridine salvage for photoassimilate allocation and partitioning.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.