ArticleThe Journal of molecular diagnostics : JMD2011
Proof of concept study to assess fetal gene expression in amniotic fluid by nanoarray PCR.
Article in The Journal of molecular diagnostics : JMD, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed, 13 citations in OpenAlex.
- Maternal obesity: sex-specific in utero changes in fetal brain autophagy and mTOR.Obesity (Silver Spring, Md.) · 2024Article
- Increased prefrontal cortex interleukin-2 protein levels and shift in the peripheral T cell population in progressive supranuclear palsy patients.Scientific reports · 2019Article
- Assessment of brain reference genes for RT-qPCR studies in neurodegenerative diseases.Scientific reports · 2016Article
- Assessing the fetal effects of maternal obesity via transcriptomic analysis of cord blood: a prospective case-control study.BJOG : an international journal of obstetrics and gynaecology · 2016Article
- The pathway not taken: understanding 'omics data in the perinatal context.American journal of obstetrics and gynecology · 2015Article
- RNA-Seq and expression microarray highlight different aspects of the fetal amniotic fluid transcriptome.Prenatal diagnosis · 2014Article
- Maternal obesity affects fetal neurodevelopmental and metabolic gene expression: a pilot study.PloS one · 2014Article
- Amniotic fluid: the use of high-dimensional biology to understand fetal well-being.Reproductive sciences (Thousand Oaks, Calif.) · 2014Review
- Tracking fetal development through molecular analysis of maternal biofluids.Biochimica et biophysica acta · 2012Review
- From prenatal genomic diagnosis to fetal personalized medicine: progress and challenges.Nature medicine · 2012Review
- The amniotic fluid transcriptome: a source of novel information about human fetal development.Obstetrics and gynecology · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 6 institutions in 1 country.
Funding
Abstract
Microarray analysis of cell-free RNA in amniotic fluid (AF) supernatant has revealed differential fetal gene expression as a function of gestational age and karyotype. Once informative genes are identified, research moves to a more focused platform such as quantitative reverse transcriptase-PCR. Standardized NanoArray PCR (SNAP) is a recently developed gene profiling technology that enables the measurement of transcripts from samples containing reduced quantities or degraded nucleic acids. We used a previously developed SNAP gene panel as proof of concept to determine whether fetal functional gene expression could be ascertained from AF supernatant. RNA was extracted and converted to cDNA from 19 AF supernatant samples of euploid fetuses between 15 to 20 weeks of gestation, and transcript abundance of 21 genes was measured. Statistically significant differences in expression, as a function of advancing gestational age, were observed for 5 of 21 genes. ANXA5, GUSB, and PPIA showed decreasing gene expression over time, whereas CASC3 and ZNF264 showed increasing gene expression over time. Statistically significantly increased expression of MTOR and STAT2 was seen in female compared with male fetuses. This study demonstrates the feasibility of focused fetal gene expression analysis using SNAP technology. In the future, this technique could be optimized to examine specific genes instrumental in fetal organ system function, which could be a useful addition to prenatal care.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.