ArticleStructure (London, England : 1993)2011
Real-time observation of strand exchange reaction with high spatiotemporal resolution.
Article in Structure (London, England : 1993), 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 76 citations in OpenAlex.
- RAD51 D-loop structures reveal the mechanism of eukaryotic RAD51-mediated strand exchange.Nature communications · 2025Article
- NProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Article
- Homology recognition without double-stranded DNA-strand separation in D-loop formation by RecA.Nucleic acids research · 2024Article
- Flanking strand separation activity of RecA nucleoprotein filaments in DNA strand exchange reactions.Nucleic acids research · 2023Article
- Construction of a Three-Color Prism-Based TIRF Microscope to Study the Interactions and Dynamics of Macromolecules.Biology · 2021Article
- Getting swept off your toe(hold)s: Single-molecule DNA fission analysis offers glimpse into kinetics of branch migration.Biophysical journal · 2021Article
- Helicase-like functions in phosphate loop containing beta-alpha polypeptides.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Micro-homology intermediates: RecA's transient sampling revealed at the single molecule level.Nucleic acids research · 2021Article
- Mismatch sensing by nucleofilament deciphers mechanism of RecA-mediated homologous recombination.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- The intrinsic ability of double-stranded DNA to carry out D-loop and R-loop formation.Computational and structural biotechnology journal · 2020Review
- Defining the influence of Rad51 and Dmc1 lineage-specific amino acids on genetic recombination.Genes & development · 2019Article
- Nonfilament-forming RecA dimer catalyzes homologous joint formation.Nucleic acids research · 2018Article
- A change of view: homologous recombination at single-molecule resolution.Nature reviews. Genetics · 2018Review
- Cooperative RecA clustering: the key to efficient homology searching.Nucleic acids research · 2017Article
- Monitoring Replication Protein A (RPA) dynamics in homologous recombination through site-specific incorporation of non-canonical amino acids.Nucleic acids research · 2017Article
- A time for promiscuity in a eukaryotic recombinase.The Journal of biological chemistry · 2017Review
- Enhancement of RecA-mediated self-assembly in DNA nanostructures through basepair mismatches and single-strand nicks.Scientific reports · 2017Article
- Cryo-EM structures of human RAD51 recombinase filaments during catalysis of DNA-strand exchange.Nature structural & molecular biology · 2017Article
- Real-time observation of DNA recognition and rejection by the RNA-guided endonuclease Cas9.Nature communications · 2016Article
Corrections and comments
- Commented on by
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
RecA binds to single-stranded (ss) DNA to form a helical filament that catalyzes strand exchange with a homologous double-stranded (ds) DNA. The study of strand exchange in ensemble assays is limited by the diffusion limited homology search process, which masks the subsequent strand exchange reaction. We developed a single-molecule fluorescence assay with a few base-pair and millisecond resolution that can separate initial docking from the subsequent propagation of joint molecule formation. Our data suggest that propagation occurs in 3 bp increments with destabilization of the incoming dsDNA and concomitant pairing with the reference ssDNA. Unexpectedly, we discovered the formation of a dynamic complex between RecA and the displaced DNA that remains bound transiently after joint molecule formation. This finding could have important implications for the irreversibility of strand exchange. Our model for strand exchange links structural models of RecA to its catalytic function.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.