ArticleThe Journal of cell biology1990
P29: a novel tyrosine-phosphorylated membrane protein present in small clear vesicles of neurons and endocrine cells.
Article in The Journal of cell biology, 1990. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 99 citations in OpenAlex.
- Phosphatidylserine-dependent structure of synaptogyrin remodels the synaptic vesicle membrane.Nature structural & molecular biology · 2023Article
- Genetic Regulation of Immunoglobulin G Glycosylation.Experientia supplementum (2012) · 2021Article
- Levetiracetam Affects Differentially Presynaptic Proteins in Rat Cerebral Cortex.Frontiers in cellular neuroscience · 2017Article
- Synaptic vesicles studied by dynamic light scattering.The European physical journal. E, Soft matter · 2011Article
- SYNGR1 is associated with schizophrenia and bipolar disorder in southern India.Journal of human genetics · 2005Article
- Fluorescence resonance energy transfer detection of synaptophysin I and vesicle-associated membrane protein 2 interactions during exocytosis from single live synapses.Molecular biology of the cell · 2002Article
- A conserved mechanism of synaptogyrin localization.Molecular biology of the cell · 2001Article
- Ion selectivities of the Ca(2+) sensors for exocytosis in rat phaeochromocytoma cells.The Journal of physiology · 2001Article
- Novel SCAMPs lacking NPF repeats: ubiquitous and synaptic vesicle-specific forms implicate SCAMPs in multiple membrane-trafficking functions.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2000Article
- Nonreceptor tyrosine protein kinase pp60c-src in spatial learning: synapse-specific changes in its gene expression, tyrosine phosphorylation, and protein-protein interactions.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Multiple and diverse forms of regulated exocytosis in wild-type and defective PC12 cells.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- SVOP, an evolutionarily conserved synaptic vesicle protein, suggests novel transport functions of synaptic vesicles.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Pantophysin is a ubiquitously expressed synaptophysin homologue and defines constitutive transport vesicles.The Journal of cell biology · 1996Article
- Structure of synaptogyrin (p29) defines novel synaptic vesicle protein.The Journal of cell biology · 1995Article
- A phosphatidylinositol 4,5-bisphosphate-sensitive casein kinase I alpha associates with synaptic vesicles and phosphorylates a subset of vesicle proteins.The Journal of cell biology · 1995Article
- Synaptobrevin binding to synaptophysin: a potential mechanism for controlling the exocytotic fusion machine.The EMBO journal · 1995Article
- Synaptic vesicle proteins and early endosomes in cultured hippocampal neurons: differential effects of Brefeldin A in axon and dendrites.The Journal of cell biology · 1993Article
- A gamma-aminobutyric acid transporter driven by a proton pump is present in synaptic-like microvesicles of pancreatic beta cells.Proceedings of the National Academy of Sciences of the United States of America · 1993Article
- The hypophyseal pars tuberalis is enriched with distinct phosphotyrosine-containing proteins not detected in other areas of the brain and pituitary.Cell and tissue research · 1993Article
- Synaptophysin-containing microvesicles transport heat-shock protein hsp60 in insulin-secreting beta cells.Cytotechnology · 1993Article
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A novel membrane protein from rat brain synaptic vesicles with an apparent 29,000 Mr (p29) was characterized. Using monospecific polyclonal antibodies, the distribution of p29 was studied in a variety of tissues by light and electron microscopy and immunoblot analysis. Within the nervous system, p29 was present in virtually all nerve terminals. It was selectively associated with small synaptic vesicles and a perinuclear region corresponding to the area of the Golgi complex. P29 was not detected in any other subcellular organelles including large dense-core vesicles. The distribution of p29 in various subcellular fractions from rat brain was very similar to that of synaptophysin and synaptobrevin. The highest enrichment occurred in purified small synaptic vesicles. Outside the nervous system, p29 was found only in endocrine cell types specialized for peptide hormone secretion. In these cells, p29 had a distribution very similar to that of synaptophysin. It was associated with microvesicles of heterogeneous size and shape that are primarily concentrated in the centrosomal-Golgi complex area. Secretory granules were mostly unlabeled, but their membrane occasionally contained small labeled evaginations. Immunoisolation of subcellular organelles from undifferentiated PC12 cells with antisynaptophysin antibodies led to a concomitant enrichment of p29, synaptobrevin, and synaptophysin, further supporting a colocalization of all three proteins. P29 has an isoelectric point of approximately 5.0 and is not N-glycosylated. It is an integral membrane protein and all antibody binding sites are exposed on the cytoplasmic side of the vesicles. Two monoclonal antibodies raised against p29 cross reacted with synaptophysin, indicating the presence of related epitopes. P29, like synaptophysin, was phosphorylated on tyrosine residues by endogenous tyrosine kinase activity in intact vesicles.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.