ArticleCell research2012
Isolation and proteomic analysis of the SYP61 compartment reveal its role in exocytic trafficking in Arabidopsis.
Article in Cell research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 114 papers.
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Who cites it
114 citing papers in PubMed.
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- Detection of Cytosolic Ion Concentrations by the Trans-Golgi Network/Early Endosome is Important for Salt Tolerance.bioRxiv : the preprint server for biology · 2025Article
- Identification of INOSITOL PHOSPHORYLCERAMIDE SYNTHASE 2 (IPCS2) as a new rate-limiting component in Arabidopsis pathogen entry control.The Plant journal : for cell and molecular biology · 2025Article
- The TRAPPC8/TRS85 subunit of the Arabidopsis TRAPPIII tethering complex regulates endoplasmic reticulum function and autophagy.Plant physiology · 2025Article
- Untangling iron threads: A deep dive into plant intracellular pools.Quantitative plant biology · 2025Review
- TYPHON proteins are RAB-dependent mediators of the trans-Golgi network secretory pathway.The Plant cell · 2024Article
- Integrated Transcriptomic, Proteomic, and Metabolomic Analyses Revealed Molecular Mechanism for Salt Resistance in Soybean (International journal of molecular sciences · 2024Article
- Protein Structural Modeling and Transport Thermodynamics Reveal That Plant Cation-Chloride Cotransporters Mediate Potassium-Chloride Symport.International journal of molecular sciences · 2024Article
- Logistics of defense: The contribution of endomembranes to plant innate immunity.The Journal of cell biology · 2024Review
- A century journey of organelles research in the plant endomembrane system.The Plant cell · 2024Review
- Phytoalexin sakuranetin attenuates endocytosis and enhances resistance to rice blast.Nature communications · 2024Article
- Breaking-Down Barriers: Proposal of Using Cellulose Biosynthesis Inhibitors and Cellulase Enzyme as a Novel Treatment Modality for Vision Threatening Pythium Insidiosum Keratitis.Clinical ophthalmology (Auckland, N.Z.) · 2024Article
- Preparation of Highly Enriched ER Membranes Using Free-Flow Electrophoresis.Methods in molecular biology (Clifton, N.J.) · 2024Article
- Tip Growth Defective1 interacts with the cellulose synthase complex to regulate cellulose synthesis in Arabidopsis thaliana.PloS one · 2024Article
- Colocalising proteins and polysaccharides in plants for cell wall and trafficking studies.Frontiers in plant science · 2024Article
- Actomyosin and CSI1/POM2 cooperate to deliver cellulose synthase from Golgi to cortical microtubules in Arabidopsis.Nature communications · 2023Article
- Nanobody-based VSR7 tracing shows clathrin-dependent TGN to Golgi recycling.Nature communications · 2023Article
- Arabidopsis flippase ALA3 is required for adjustment of early subcellular trafficking in plant response to osmotic stress.Journal of experimental botany · 2023Article
- Multiple host targets ofbioRxiv : the preprint server for biology · 2023Article
54 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The endomembrane system is a complex and dynamic intracellular trafficking network. It is very challenging to track individual vesicles and their cargos in real time; however, affinity purification allows vesicles to be isolated in their natural state so that their constituent proteins can be identified. Pioneering this approach in plants, we isolated the SYP61 trans-Golgi network compartment and carried out a comprehensive proteomic analysis of its contents with only minimal interference from other organelles. The proteome of SYP61 revealed the association of proteins of unknown function that have previously not been ascribed to this compartment. We identified a complete SYP61 SNARE complex, including regulatory proteins and validated the proteome data by showing that several of these proteins associated with SYP61 in planta. We further identified the SYP121-complex and cellulose synthases, suggesting that SYP61 plays a role in the exocytic trafficking and the transport of cell wall components to the plasma membrane. The presence of proteins of unknown function in the SYP61 proteome including ECHIDNA offers the opportunity to identify novel trafficking components and cargos. The affinity purification of plant vesicles in their natural state provides a basis for further analysis and dissection of complex endomembrane networks. The approach is widely applicable and can afford the study of several vesicle populations in plants, which can be compared with the SYP61 vesicle proteome.
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