ArticleProceedings of the National Academy of Sciences of the United States of America2011
High-level recombinant protein expression in transgenic plants by using a double-inducible viral vector.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
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Who cites it
46 citing papers in PubMed, 87 citations in OpenAlex.
- Article
- Plant-Based Systems for Producing Therapeutic Proteins: Current Status and Future Prospects.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2026Review
- Review
- Engineering Conditional Transgene Expression in Nicotiana benthamiana.Plant biotechnology journal · 2026Review
- CuBe: a geminivirus-based copper-regulated expression system suitable for post-harvest activation.Plant biotechnology journal · 2025Article
- Trans-complementation of the viral movement protein mediates efficient expression of large target genes via a tobacco mosaic virus vector.Plant biotechnology journal · 2024Article
- Plant-made pharmaceuticals.Plant biotechnology (Tokyo, Japan) · 2024Article
- The design of synthetic gene circuits in plants: new components, old challenges.Journal of experimental botany · 2023Review
- Facilitating viral vector movement enhances heterologous protein production in an established plant system.Plant biotechnology journal · 2023Article
- Functional characterization of VirB/VirD4 and Icm/Dot type IV secretion systems from the plant-pathogenic bacteriumFrontiers in cellular and infection microbiology · 2023Article
- Maximizing the Production of Recombinant Proteins in Plants: From Transcription to Protein Stability.International journal of molecular sciences · 2022Review
- Recent Progress on Vaccines Produced in Transgenic Plants.Vaccines · 2022Review
- Expression strategies for the efficient synthesis of antimicrobial peptides in plastids.Nature communications · 2022Article
- Development of plant-made monoclonal antibodies against viral infections.Current opinion in virology · 2022Review
- Production of Recombinant Active Human TGFβ1 inFrontiers in plant science · 2022Article
- Improving Recombinant Protein Recovery from Plant Tissue Using Heat Precipitation.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Expanding the application of a UV-visible reporter for transient gene expression and stable transformation in plants.Horticulture research · 2021Article
- Techno-economic process modelling and Monte Carlo simulation data of uncertainty quantification in field-grown plant-based manufacturing.Data in brief · 2021Article
- Process Simulation and Techno-Economic Analysis of Large-Scale Bioproduction of Sweet Protein Thaumatin II.Foods (Basel, Switzerland) · 2021Article
- Techno-economic analysis of a plant-based platform for manufacturing antimicrobial proteins for food safety.Biotechnology progress · 2020Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We describe here a unique ethanol-inducible process for expression of recombinant proteins in transgenic plants. The process is based on inducible release of viral RNA replicons from stably integrated DNA proreplicons. A simple treatment with ethanol releases the replicon leading to RNA amplification and high-level protein production. To achieve tight control of replicon activation and spread in the uninduced state, the viral vector has been deconstructed, and its two components, the replicon and the cell-to-cell movement protein, have each been placed separately under the control of an inducible promoter. Transgenic Nicotiana benthamiana plants incorporating this double-inducible system demonstrate negligible background expression, high (over 0.5 × 10(4)-fold) induction multiples, and high absolute levels of protein expression upon induction (up to 4.3 mg/g fresh biomass). The process can be easily scaled up, supports expression of practically important recombinant proteins, and thus can be directly used for industrial manufacturing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.