ArticleNucleic acids research2011
Cell type specific, traceable gene silencing for functional gene analysis during vertebrate neural development.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
- miR-219a-Robo1 axis acts as a conserved switch for slit-mediated commissural axon guidance.iScience · 2025Article
- A cell-autonomous role for primary cilium-mediated signaling in long-range commissural axon guidance.Development (Cambridge, England) · 2024Article
- 3D exploration of gene expression in chicken embryos through combined RNA fluorescence in situ hybridization, immunofluorescence, and clearing.BMC biology · 2024Article
- The role of microtubule-associated protein tau in netrin-1 attractive signaling.Journal of cell science · 2024Article
- Cables1 links Slit/Robo and Wnt/Frizzled signaling in commissural axon guidance.Development (Cambridge, England) · 2023Article
- Endoglycan Regulates Purkinje Cell Migration by Balancing Cell-Cell Adhesion.Frontiers in neuroscience · 2022Article
- Axon guidance at the spinal cord midline-A live imaging perspective.The Journal of comparative neurology · 2021Article
- Investigating Primary Cilia during Peripheral Nervous System Formation.International journal of molecular sciences · 2021Article
- Article
- Motor neurons control blood vessel patterning in the developing spinal cord.Nature communications · 2017Article
- Sonic -'Jack-of-All-Trades' in Neural Circuit Formation.Journal of developmental biology · 2017Review
- Canonical wnt signaling is required for commissural axon guidance.Developmental neurobiology · 2016Article
- A 'tool box' for deciphering neuronal circuits in the developing chick spinal cord.Nucleic acids research · 2014Article
- Semaphorin 6B acts as a receptor in post-crossing commissural axon guidance.Development (Cambridge, England) · 2014Article
- Development of retroviral vectors for tissue-restricted expression in chicken embryonic gonads.PloS one · 2014Article
- In ovo electroporation of miRNA-based plasmids in the developing neural tube and assessment of phenotypes by DiI injection in open-book preparations.Journal of visualized experiments : JoVE · 2012Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many genes have several, sometimes divergent functions during development. Therefore, timing of gene knockdown for functional analysis during development has to be done with precise temporal control, as loss of a gene's function at early stages prevents its analysis later in development. RNAi, in combination with the accessibility of chicken embryos, is an effective approach for temporally controlled analysis of gene function during neural development. Here, we describe novel plasmid vectors that contain cell type-specific promoters/enhancers to drive the expression of a fluorescent marker, followed directly by a miR30-RNAi transcript for gene silencing. These vectors allow for direct tracing of cells experiencing gene silencing by the bright fluorescence. The level of knockdown is sufficient to reproduce the expected pathfinding defects upon perturbation of genes with known axon guidance functions. Mixing different vectors prior to electroporation enables the simultaneous knockdown of multiple genes in independent regions of the spinal cord. This permits complex cellular and molecular interactions to be examined during development, in a fast and precise manner. The advancements of the in ovo RNAi technique that we describe will not only markedly enhance functional gene analysis in the chicken, but also could be adapted to other organisms in developmental studies.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.