ArticleNucleic acids research2011
Impartial comparative analysis of measurement of leukocyte telomere length/DNA content by Southern blots and qPCR.
Article in Nucleic acids research, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 211 papers, 16 of them syntheses that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
211 citing papers in PubMed, 16 syntheses or guidelines pooled it, 342 citations in OpenAlex.
- Exploring the association between depression and telomere length: A systematic review and meta-analysis.Scientific reports · 2025Pooled it
- Shorter telomere length as a prognostic marker for survival and recurrence in breast cancer: a systematic review and meta-analysis.Exploration of targeted anti-tumor therapy · 2025Pooled it
- Validation of human telomere length multi-ancestry meta-analysis association signals identifies POP5 and KBTBD6 as human telomere length regulation genes.Nature communications · 2024Pooled it
- A meta-analysis on the heritability of vertebrate telomere length.Journal of evolutionary biology · 2022Pooled it
- A systematic review and meta-analysis of the Everyday Discrimination Scale and biomarker outcomes.Psychoneuroendocrinology · 2022Pooled it
- Biological aging in childhood and adolescence following experiences of threat and deprivation: A systematic review and meta-analysis.Psychological bulletin · 2020Pooled it
- Telomere length in granulosa cells and leukocytes: a potential marker of female fertility? A systematic review of the literature.Journal of ovarian research · 2020Pooled it
- Loci for human leukocyte telomere length in the Singaporean Chinese population and trans-ethnic genetic studies.Nature communications · 2019Pooled it
- The relationship between telomere length and mortality risk in non-model vertebrate systems: a meta-analysis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2018Pooled it
- Cigarette smoking and telomere length: A systematic review of 84 studies and meta-analysis.Environmental research · 2017Pooled it
- The impact of hypertension on leukocyte telomere length: a systematic review and meta-analysis of human studies.Journal of human hypertension · 2017Pooled it
- Telomere length in Parkinson's disease: A meta-analysis.Experimental gerontology · 2016Pooled it
- DCAF4, a novel gene associated with leucocyte telomere length.Journal of medical genetics · 2015Pooled it
- Gender and telomere length: systematic review and meta-analysis.Experimental gerontology · 2014Pooled it
- Telomere length and physical performance at older ages: an individual participant meta-analysis.PloS one · 2013Pooled it
- Is socioeconomic status associated with biological aging as measured by telomere length?Epidemiologic reviews · 2013Pooled it
- Nested case-control study of telomere length and lung cancer risk among heavy smokers in the β-Carotene and Retinol Efficacy Trial.British journal of cancer · 2018Trial
- Accelerated telomere shortening: Tracking the lasting impact of early institutional care at the cellular level.Psychiatry research · 2016Trial
- Independent and combined effects of dietary weight loss and exercise on leukocyte telomere length in postmenopausal women.Obesity (Silver Spring, Md.) · 2013Trial
- Association of TERC and OBFC1 haplotypes with mean leukocyte telomere length and risk for coronary heart disease.PloS one · 2013Trial
151 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 4 institutions in 1 country.
Funding
Abstract
Telomere length/DNA content has been measured in epidemiological/clinical settings with the goal of testing a host of hypotheses related to the biology of human aging, but often the conclusions of these studies have been inconsistent. These inconsistencies may stem from various reasons, including the use of different telomere length measurement techniques. Here, we report the first impartial evaluation of measurements of leukocyte telomere length by Southern blot of the terminal restriction fragments and quantitative PCR (qPCR) of telomere DNA content, expressed as the ratio of telomeric product (T)/single copy gene (S) product. Blind measurements on the same samples from 50 donors were performed in two independent laboratories on two different occasions. Both the qPCR and Southern blots displayed highly reproducible results as shown by r values > 0.9 for the correlations between results obtained by either method on two occasions. The inter-assay CV measurement for the qPCR was 6.45%, while that of the Southern blots was 1.74%. The relation between the results generated by Southern blots versus those generated by qPCR deviated from linearity. We discuss the ramifications of these findings with regard to measurements of telomere length/DNA content in epidemiological/clinical circumstances.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.