ArticleAging cell2011
Age-related decline in osteoblastogenesis and 1α-hydroxylase/CYP27B1 in human mesenchymal stem cells: stimulation by parathyroid hormone.
Article in Aging cell, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 51 citations in OpenAlex.
- Acute Effects of Kisspeptin Administration on Bone Metabolism in Healthy Men.The Journal of clinical endocrinology and metabolism · 2022Trial
- Review
- Synergistic stimulation of osteoblast differentiation of rat mesenchymal stem cells by leptin and 25(OH)DStem cell research & therapy · 2021Article
- Parathyroid hormone promotes the osteogenesis of lipopolysaccharide-induced human bone marrow mesenchymal stem cells through the JNK MAPK pathway.Bioscience reports · 2021Article
- Loss of KDM4B exacerbates bone-fat imbalance and mesenchymal stromal cell exhaustion in skeletal aging.Cell stem cell · 2021Article
- A Novel Peptide, CK2.3, Improved Bone Formation in Ovariectomized Sprague Dawley Rats.International journal of molecular sciences · 2020Article
- 1,25-Dihydroxyvitamin D insufficiency accelerates age-related bone loss by increasing oxidative stress and cell senescence.American journal of translational research · 2020Article
- The Regulation of Marrow Fat by Vitamin D: Molecular Mechanisms and Clinical Implications.Current osteoporosis reports · 2019Review
- Vitamin D Metabolism Revised: Fall of Dogmas.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2019Review
- Physiologic and pathophysiologic roles of extra renal CYP27b1: Case report and review.Bone reports · 2018Article
- Adrenocorticotropic hormone and 1,25-dihydroxyvitamin DLaboratory investigation; a journal of technical methods and pathology · 2017Article
- Chronic kidney disease and vitamin D metabolism in human bone marrow-derived MSCs.Annals of the New York Academy of Sciences · 2017Review
- Parathyroid Hormone-Induced Bone Marrow Mesenchymal Stem Cell Chondrogenic Differentiation and its Repair of Articular Cartilage Injury in Rabbits.Medical science monitor basic research · 2016Article
- Regulation of CYP27B1 mRNA Expression in Primary Human Osteoblasts.Calcified tissue international · 2016Article
- Transplantation of bone marrow-derived mesenchymal stem cells rescues partially rachitic phenotypes induced by 1,25-Dihydroxyvitamin D deficiency in mice.American journal of translational research · 2016Article
- Paracrine effects of haematopoietic cells on human mesenchymal stem cells.Scientific reports · 2015Article
- Effect of age on regulation of human osteoclast differentiation.Journal of cellular biochemistry · 2014Article
- Vitamin D and gene networks in human osteoblasts.Frontiers in physiology · 2014Review
- Effects of parathyroid hormone on calcium ions in rat bone marrow mesenchymal stem cells.BioMed research international · 2014Article
- Vitamin D endocrine system and osteoblasts.BoneKEy reports · 2014Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 2 countries.
Funding
Abstract
With aging, there is a decline in bone mass and in osteoblast differentiation of human mesenchymal stem cells (hMSCs) in vitro. Osteoblastogenesis can be stimulated with 1,25-dihydroxyvitamin D(3) [1,25(OH)(2) D(3) ] and, in some hMSCs, by the precursor 25-hydroxyvitamin D(3) (25OHD(3) ). CYP27B1/1α-hydroxylase activates 25OHD(3) and, to a variable degree, hMSCs express CYP27B1. In this study, we tested the hypotheses (i) that age affects responsiveness to 25OHD(3) and expression/activity of CYP27B1 in hMSCs and (ii) that parathyroid hormone (PTH) upregulates CYP27B1 in hMSCs, as it does in renal cells. There were age-related declines in osteoblastogenesis (n=8, P=0.0286) and in CYP27B1 gene expression (n=27, r= -0.498; P=0.008) in hMSCs. Unlike hMSCs from young subjects (≤50 years), hMSCs from older subjects (≥55 years) were resistant to 25OHD(3) stimulation of osteoblastogenesis. PTH1-34 (100 nm) provided hMSCs with responsiveness to 25OHD(3) (P=0.0313, Wilcoxon matched pairs test) and with two episodes of increased 1,25(OH)(2) D(3) synthesis, of cAMP response element binding protein (CREB) activation, and of CYP27B1 upregulation. Both increases in CYP27B1 expression by PTH were obliterated by CREB-siRNA or KG-501 (which specifically inhibits the downstream binding of activated CREB). Only the second period of CREB signaling was diminished by AG1024, an inhibitor of insulin-like growth factor-I receptor kinase. Thus, PTH stimulated hMSCs from elders with responsiveness to 25OHD(3) by upregulating expression/activity of CYP27B1 and did so through CREB and IGF-I pathways.
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