ArticleInternational journal of microbiology2011
Populations of select cultured and uncultured bacteria in the rumen of sheep and the effect of diets and ruminal fractions.
Article in International journal of microbiology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- Enhancing inference of differential gene expression in metatranscriptomes from human microbial communities.Nature communications · 2026Article
- Enhancing the fiber degradation efficiency in dairy cattle rumen through engineered bacterial communities.Microbiome · 2026Article
- Effects of Rumen-Protected Methionine and Lysine on the Fecal Microbiota of Leizhou Goats.Microorganisms · 2025Article
- Systematic evaluation of metatranscriptomic differential gene expressionbioRxiv : the preprint server for biology · 2025Article
- Multi-omics analysis of the mechanism of alfalfa and wheat-induced rumen flatulence in Xizang sheep.Microbiology spectrum · 2025Article
- Changes in Rumen Microbiology and Metabolism of Tibetan Sheep with Different Lys/Met Ratios in Low-Protein Diets.Animals : an open access journal from MDPI · 2024Article
- Article
- Rumen-Protected Lysine and Methionine Supplementation Reduced Protein Requirement of Holstein Bulls by Altering Nitrogen Metabolism in Liver.Animals : an open access journal from MDPI · 2023Article
- Ruminal bacterial community is associated with the variations of total milk solid content in Holstein lactating cows.Animal nutrition (Zhongguo xu mu shou yi xue hui) · 2022Article
- Effect of barley straw and Egyptian clover hay on the rumen fermentation and structure and fibrolytic activities of rumen bacteria in dromedary camel.Veterinary world · 2022Article
- Effects of Dietary Antimicrobial Growth Promoters on Performance Parameters and Abundance and Diversity of Broiler Chicken Gut Microbiome and Selection of Antibiotic Resistance Genes.Frontiers in microbiology · 2022Article
- Inhibition of Rumen Protozoa by Specific Inhibitors of Lysozyme and PeptidasesFrontiers in microbiology · 2019Article
- Modifications and optimization of manual methods for polymerase chain reaction and 16S rRNA gene sequencing quality community DNA extraction from goat rumen digesta.Veterinary world · 2018Article
- Does intra-ruminal nitrogen recycling waste valuable resources? A review of major players and their manipulation.Journal of animal science and biotechnology · 2018Review
- Isolation and identification of a cellulolyticVeterinary world · 2017Article
- Metagenomic investigation of gastrointestinal microbiome in cattle.Asian-Australasian journal of animal sciences · 2017Review
- Characterization of faecal microbial communities of dairy cows fed diets containing ensiled Moringa oleifera fodder.Scientific reports · 2017Article
- Use of Lysozyme as a Feed Additive onAsian-Australasian journal of animal sciences · 2016Article
- Dietary supplementation of Rosmarinus officinalis L. leaves in sheep affects the abundance of rumen methanogens and other microbial populations.Journal of animal science and biotechnology · 2016Article
- First insights into the microbial diversity in the omasum and reticulum of bovine using Illumina sequencing.Journal of applied genetics · 2015Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The objective of this study was to assess the importance of select cultured and uncultured bacteria in the rumen by quantifying their populations and the effect of diets and ruminal fractions. Full-length 16S rRNA gene (rrs) sequences were recovered from rumen samples using specific primers designed from partial sequences recovered previously. Five uncultured bacterial operational taxonomic units (OTUs) were quantified using specific quantitative PCR (qPCR) in fractionated ruminal samples from sheep fed either hay alone or hay plus corn. Species Fibrobacter succinogenes, Ruminococcus albus, R. flavefaciens, Ruminobacter amylophilus, Selenomonas ruminantium, and Mitsuokella multacida and genera Butyrivibrio and Prevotella were also quantified as comparison. The full-length rrs sequence improved taxonomic assignments of partial rrs sequences. Genus Prevotella had the greatest abundance. Of the three major cultured cellulolytic species, R. flavefaciens was most abundant, followed by R. albus and F. succinogenes. The five uncultured bacterial OTUs, classified to genus Acetivibrio, genus Allobaculum, family Ruminococcaceae, order Clostridiales, or class Clostridia, had abundance comparable to that of the above species of genera except Prevotella. Corn supplementation and fractions affected distribution of the rumen bacteria, but to a limited extent. When compared to the qPCR data, sequence frequencies in the rrs clone libraries tended to overestimate the abundance of the bacteria represented. This study showed that abundance and population dynamics of uncultured bacteria can be quantified by specific qPCR, which complements the results of rrs clone libraries. This study also revealed that some uncultured bacteria might be as important as some of the well-characterized bacteria in the rumen. The approach used should be applicable to assess the abundance and potential importance of uncultured bacteria in other environments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.