ArticleNature biotechnology2011
Quantitative fluorescence imaging of protein diffusion and interaction in living cells.
Article in Nature biotechnology, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
73 citing papers in PubMed, 153 citations in OpenAlex.
- Fluorescence Correlation Spectroscopy to Examine Protein-Lipid Interactions in Membranes.Methods in molecular biology (Clifton, N.J.) · 2026Article
- HBimmCue: A Versatile Fluorescent Probe for Multi-Scale Imaging of Lipid Polarity and Membrane Order in Inner Mitochondrial Membrane.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- A practical guide to light-sheet microscopy for nanoscale imaging: Looking beyond the cell.Journal of applied physics · 2024Article
- Segmented fluorescence correlation spectroscopy (FCS) on a commercial laser scanning microscope.Scientific reports · 2024Article
- Plasmonic random laser enabled artefact-free wide-field fluorescence bioimaging: uncovering finer cellular features.Nanoscale advances · 2022Article
- How Genomes Emerge, Function, and Evolve: Living Systems Emergence-Genotype-Phenotype-Multilism-Genome/Systems Ecology.Results and problems in cell differentiation · 2022Article
- Simulation of Different Three-Dimensional Models of Whole Interphase Nuclei Compared to Experiments - A Consistent Scale-Bridging Simulation Framework for Genome Organization.Results and problems in cell differentiation · 2022Article
- Scaling Relationship in Chromatin as a Polymer.Results and problems in cell differentiation · 2022Article
- A survey of physical methods for studying nuclear mechanics and mechanobiology.APL bioengineering · 2021Review
- Nanowaveguide-illuminated fluorescence correlation spectroscopy for single molecule studies.AIP advances · 2021Article
- Degradation of Drug Delivery Nanocarriers and Payload Release: A Review of Physical Methods for Tracing Nanocarrier Biological Fate.Pharmaceutics · 2021Review
- Confocal Laser Scanning Microscopy and Fluorescence Correlation Methods for the Evaluation of Molecular Interactions.Advances in experimental medicine and biology · 2021Article
- Application of quantitative cell imaging using label-free optical diffraction tomography.Biophysics and physicobiology · 2021Article
- Of numbers and movement - understanding transcription factor pathogenesis by advanced microscopy.Disease models & mechanisms · 2020Review
- Fast Diffusion of the Unassembled PetC1-GFP Protein in the Cyanobacterial Thylakoid Membrane.Life (Basel, Switzerland) · 2020Article
- Phase separation at the nanoscale quantified by dcFCCS.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Combined Selective Plane Illumination Microscopy and FRAP Maps Intranuclear Diffusion of NLS-GFP.Biophysical journal · 2020Article
- Fluorescence strategies for mapping cell membrane dynamics and structures.APL bioengineering · 2020Article
- Light-Sheet Microscopy for Surface Topography Measurements and Quantitative Analysis.Sensors (Basel, Switzerland) · 2020Article
- Dynamic Imaging of Transferrin Receptor Molecules on Single Live Cell with Bridge Gaps-Enhanced Raman Tags.Nanomaterials (Basel, Switzerland) · 2019Article
13 more citing papers are in PubMed but not listed here.
Corrections and comments
- Commented on by
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Diffusion processes and local dynamic equilibria inside cells lead to nonuniform spatial distributions of molecules, which are essential for processes such as nuclear organization and signaling in cell division, differentiation and migration. To understand these mechanisms, spatially resolved quantitative measurements of protein abundance, mobilities and interactions are needed, but current methods have limited capabilities to study dynamic parameters. Here we describe a microscope based on light-sheet illumination that allows massively parallel fluorescence correlation spectroscopy (FCS) measurements and use it to visualize the diffusion and interactions of proteins in mammalian cells and in isolated fly tissue. Imaging the mobility of heterochromatin protein HP1α (ref. 4) in cell nuclei we could provide high-resolution diffusion maps that reveal euchromatin areas with heterochromatin-like HP1α-chromatin interactions. We expect that FCS imaging will become a useful method for the precise characterization of cellular reaction-diffusion processes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.