Evidence map›Paper›PMID 21821905›Full record

ArticleActa crystallographica. Section F, Structural biology and crystallization communications2011

Expression, purification and preliminary crystallographic analysis of O-acetylhomoserine sulfhydrylase from Mycobacterium tuberculosis.

Jiang Yin, Craig R Garen, Katherine Bateman, Minmin Yu, Emily Z Alipio Lyon, Jeff Habel, Heungbok Kim, Li-wei Hung, Chang-Yub Kim, Michael N G James

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Acta crystallographica. Section F, Structural biology and crystallization communications, 2011. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.1field-weighted citation impact, top 47% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 3 institutions in 2 countries.

Jiang YinDepartment of Biochemistry, School of Molecular and Systems Medicine, Faculty of Medicine and Dentistry, University of Alberta, Edmonton, Alberta, Canada.
Craig R Garen
Katherine Bateman
Minmin Yu
Emily Z Alipio Lyon
Jeff Habel
Heungbok Kim
Li-wei Hung
Chang-Yub Kim
Michael N G James
University of Alberta · CALawrence Berkeley National Laboratory · USLos Alamos National Laboratory · US

Funding

Canadian Institutes of Health Research
6 · The paper itself

Abstract

The gene product of the open reading frame Rv3340 from Mycobacterium tuberculosis is annotated as encoding a probable O-acetylhomoserine (OAH) sulfhydrylase (MetC), an enzyme that catalyzes the last step in the biosynthesis of methionine, which is an essential amino acid in bacteria and plants. Following overexpression in Escherichia coli, the M. tuberculosis MetC enzyme was purified and crystallized using the hanging-drop vapor-diffusion method. Native diffraction data were collected from crystals belonging to space group P2(1) and were processed to a resolution of 2.1 Å.

Indexed as

Amino Acid SequenceCarbon-Oxygen LyasesConserved SequenceCrystallography, X-RayGene ExpressionHumansMolecular Sequence DataMycobacterium tuberculosisSequence AlignmentCarbon-Oxygen LyasesO-acetylhomoserine (thiol)-lyase

Identifiers

PMID21821905
PMCPMC3151138
OpenAlexW2031229676

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.